Genetic interactions of seprase regulatory subunits reveal the diverged Drosophila Cenp-C homolog
Genetic interactions of seprase regulatory subunits reveal the diverged Drosophila Cenp-C homolog
复制标题
DOI：
10.1101/gad.347805
复制
发表时间：
2005-09-01
影响因子：
10.5
通讯作者：
Lehner, CF
中科院分区：
文献类型：
--
作者：
Heeger, S;Leismann, O;Lehner, CF
在有丝分裂过程中遗传信息的忠实传递依赖于姐妹动粒与有丝分裂纺锤体的双极连接，以及在中期到后期转变前夕姐妹染色单体粘连的完全解除。分离酶被认为负责姐妹染色单体的分离，但其调控机制尚未完全清楚。因此，我们筛选了能改变果蝇中调节性分离酶复合亚基Pimples/securin和Three rows过表达所导致的异常表型的基因位点。发现一个相互作用的基因编码一种组成型着丝粒蛋白。对其着丝粒定位结构域的特征分析揭示了一个分化的CENPC基序的存在。虽然无法获得该果蝇Cenp - C同源物参与着丝粒处分离酶激活的直接证据，但体内成像清楚地表明它是动粒正常连接到纺锤体所必需的。
Faithful transmission of genetic information during mitotic divisions depends on bipolar attachment of sister kinetochores to the mitotic spindle and on complete resolution of sister-chromatid cohesion immediately before the metaphase-to-anaphase transition. Separase is thought to be responsible for sister-chromatid separation, but its regulation is not completely understood. Therefore, we have screened for genetic loci that modify the aberrant phenotypes caused by overexpression of the regulatory separase complex subunits Pimples/securin and Three rows in Drosophila. An interacting gene was found to encode a constitutive centromere protein. Characterization of its centromere localization domain revealed the presence of a diverged CENPC motif. While direct evidence for an involvement of this Drosophila Cenp-C homolog in separase activation at centromeres could not be obtained, in vivo imaging clearly demonstrated that it is required for normal attachment of kinetochores to the spindle.