The gel state of the vitreous and ascorbate-dependent oxygen consumption: relationship to the etiology of nuclear cataracts.
The gel state of the vitreous and ascorbate-dependent oxygen consumption: relationship to the etiology of nuclear cataracts.
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DOI：
10.1001/archophthalmol.2008.621
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发表时间：
2009-04
影响因子：
--
通讯作者：
Beebe, David C.
中科院分区：
文献类型：
--
作者：
Shui, Ying-Bo;Holekamp, Nancy M.;Kramer, Benjamin C.;Crowley, Jan R.;Wilkins, Mark A.;Chu, Fred;Malone, Paula E.;Mangers, Shayna J.;Hou, Joshua H.;Siegfried, Carla J.;Beebe, David C.
研究玻璃体耗氧的速率和机制。用微呼吸计测量耗氧量。通过分光光度法和气相色谱-质谱法测量玻璃体抗坏血酸。玻璃体变性与玻璃体切除术过程中获得的样品中的耗氧率和抗坏血酸浓度有关。长时间接触氧气或用抗坏血酸氧化酶治疗可消除玻璃体的耗氧量。添加抗坏血酸可恢复耗氧量。煮沸或用螯合剂 EDTA 和去铁胺处理玻璃体后，耗氧量仍然存在。在接受视网膜手术的患者中，玻璃体液化和先前的玻璃体切除术与抗坏血酸浓度降低和耗氧量降低有关。玻璃体中的抗坏血酸可减少晶状体与氧气的接触。尽管游离铁可能有所贡献，但该反应的催化剂尚不清楚。玻璃体的凝胶状态保持抗坏血酸水平，从而维持氧气消耗。玻璃体切除术或晚期玻璃体变性可能会增加晶状体与氧气的接触，促进核性白内障的进展。确定如何保护眼睛免受核性白内障的影响应该提出减少核性白内障发病率的治疗方法。
To investigate the rate and mechanism of oxygen consumption by the vitreous. Oxygen consumption was measured with a microrespirometer. Vitreous ascorbate was measured spectrophotometrically and by gas chromatography–mass spectrometry. Vitreous degeneration was related to the rate of oxygen consumption and ascorbate concentration in samples obtained during vitrectomy. Prolonged exposure to oxygen or treatment with ascorbate oxidase eliminated oxygen consumption by the vitreous. Adding ascorbate restored oxygen consumption. Oxygen consumption persisted after boiling or treating the vitreous with the chelating agents EDTA and deferoxamine. In patients undergoing retinal surgery, liquefaction of the vitreous and previous vitrectomy were associated with decreased ascorbate concentration and lower oxygen consumption. Ascorbate in the vitreous decreases exposure of the lens to oxygen. The catalyst for this reaction is not known, although free iron may contribute. The gel state of the vitreous preserves ascorbate levels, thereby sustaining oxygen consumption. Vitrectomy or advanced vitreous degeneration may increase exposure of the lens to oxygen, promoting the progression of nuclear cataracts. Determining how the eye is protected from nuclear cataracts should suggest treatments to reduce their incidence.
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影响因子：
4.2
作者：
DEBUSTROS, S;THOMPSON, JT;GLASER, BM
通讯作者：
GLASER, BM
影响因子：
--
作者：
Kubo, E.;Kumamoto, Y.;Akagi, Y.
通讯作者：
Akagi, Y.
影响因子：
4.2
作者：
Holekamp, NM;Shui, YB;Beebe, DC
通讯作者：
Beebe, DC
影响因子：
3.1
作者：
BUERK, DG;SHONAT, RD;CRANSTOUN, SD
通讯作者：
CRANSTOUN, SD
影响因子：
2.8
作者：
Chen, SN;Lin, KK;Ho, JD
通讯作者：
Ho, JD