Heterogeneity of T Cell Responses to Pandemic pH1N1 Monovalent Vaccine in HIV-Infected Pregnant Women.
Heterogeneity of T Cell Responses to Pandemic pH1N1 Monovalent Vaccine in HIV-Infected Pregnant Women.
复制标题
HIV 感染孕妇 T 细胞对流行性 pH1N1 单价疫苗反应的异质性。
DOI：
10.1089/aid.2015.0151
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发表时间：
2015
影响因子：
1.5
通讯作者：
P1086StudyTeam
中科院分区：
文献类型：
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作者：
Weinberg,Adriana;Muresan,Petronella;Richardson,Kelly;Fenton,Terence;Dominguez,Teresa;Bloom,Anthony;Watts,DHeather;Abzug,MarkJ;Nachman,SharonA;Levin,MyronJ;P1086StudyTeam
我们研究了接受联合抗逆转录病毒治疗（cART）的HIV感染孕妇对pH 1 N1单价流感疫苗（IIV 1）的Th 1保护性和调节性T和B细胞（Treg和布雷格）应答。52名研究参与者的外周血单核细胞（PBMC）在接种疫苗前后冷冻保存，并通过流式细胞术进行分析。用pH 1 N1和对照抗原体外刺激后，在PBMC中测量pH 1 N1特异性Th 1、Treg和布雷格应答。队列分析未检测到接种后pH 1 N1-Th 1、Treg或布雷格亚群的变化。然而，个体分析区分了接种疫苗后产生强烈Th 1应答的受试者和未产生强烈Th 1应答的受试者。疫苗接种后，高pH 1 N1-Th 1与高pH 1 N1-Treg和布雷格应答相关，表明低流感效应子应答不是由过度疫苗诱导的免疫调节引起的。高接种后pH 1 N1-Th 1应答与基线高PHA-和pH 1 N1-IFN-γ ELISpot和循环CD 4 + CD 39 +%和CD 8 + CD 39 +% Treg相关，与低CD 8+细胞数和CD 19 + FOXP 3 +%布雷格相关，但与CD 4+细胞数或HIV病毒载量无关。这些数据突出了接受cART的HIV感染者中T细胞对疫苗应答的异质性。Th 1对IIV的强应答的预测因子包括CD 8+细胞数量、T细胞功能和循环布雷格和Treg。
We investigated the Th1 protective and regulatory T and B cell (Treg and Breg) responses to pH1N1 monovalent influenza vaccine (IIV1) in HIV-infected pregnant women on combination antiretroviral therapy (cART). Peripheral blood mononuclear cells (PBMCs) from 52 study participants were cryopreserved before and after vaccination and analyzed by flow cytometry. pH1N1-specific Th1, Treg, and Breg responses were measured in PBMCs afterin vitrostimulation with pH1N1 and control antigen. The cohort analysis did not detect changes in pH1N1-Th1, Treg, or Breg subsets postvaccination. However, individual analyses distinguished subjects who mounted vigorous Th1 responses postvaccination from others who did not. Postvaccination, high pH1N1-Th1 correlated with high pH1N1-Treg and Breg responses, suggesting that low influenza effector responses did not result from excessive vaccine-induced immune regulation. High postvaccination pH1N1-Th1 responses correlated with baseline high PHA- and pH1N1-IFN-γ ELISpot and circulating CD4+CD39+% and CD8+CD39+% Treg, with low CD8+cell numbers and CD19+FOXP3+% Breg, but not with CD4+cell numbers or HIV viral load. These data highlight the heterogeneity of T cell responses to vaccines in HIV-infected individuals on cART. Predictors of robust Th1 responses to IIV include CD8+cell numbers, T cell functionality, and circulating Breg and Treg.