Exploring the Toxicity of a Bismuth-Asparagine Coordination Polymer on the Early Development of Zebrafish Embryos
Exploring the Toxicity of a Bismuth-Asparagine Coordination Polymer on the Early Development of Zebrafish Embryos
复制标题
探索铋-天冬酰胺配位聚合物对斑马鱼胚胎早期发育的毒性
DOI：
10.1021/tx3004032
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发表时间：
2013-01-01
影响因子：
4.1
通讯作者：
Feng, Xizeng
中科院分区：
文献类型：
--
作者：
He, Ningning;Li, Xiang;Feng, Xizeng
纳米颗粒被广泛应用于纳米医学，这引发了人们对其毒性的担忧。本研究评价了天冬酰胺铋配位聚合物微球(BACP-2)对斑马鱼胚胎的毒性。注射BACP-2的1-4细胞期胚胎导致头部较小(尤其是眼睛较小)，体长较短，并出现心包水肿。由此产生的表型的严重程度和发生情况与浓度有关。Krox20、同源异型盒2(OTX2)和心肌肌球蛋白轻链2(Cmlc2)等基因的表达表明，BACP-2对头部和心脏的影响与基因表达模式的改变有关。外胚期的延迟被观察到，并且NO尾(NTL)基因的表达水平表明外胚期的延迟既是由于BACP-2对外胚期细胞迁移的影响，也是由于生长缓慢所致。这些发现表明，BACP-2表现出浓度依赖的发育毒性，为了解铋衍生物的纳米毒性提供了洞察力，在将其应用于纳米医学之前，必须严格评估其毒性。
Nanoparticles are widely used in nanomedicine, raising concerns about their toxicity. In this study, the toxicity of bismuth asparagine coordination polymer spheres (BACP-2) was assessed in zebrafish embryos. Injection of 1-4 cell stage embryos with BACP-2 resulted in smaller head size (particularly smaller eye size), shorter body length, and pericardial edemas. The severity and occurrence of the resulting phenotype were concentration-dependent. The expression of genes such as krox20, orthodenticle homeobox 2 (otx2), and cardiac myosin light chain-2 (cmlc2) indicates that the effects of BACP-2 on the head and heart were related to changes in gene expression patterns. A delay in epiboly was observed, and the expression levels of the no tail (ntl) gene indicated that the delay in epiboly resulted both from the effect of BACP-2 on cell migration during epiboly and from slow growth. These findings indicate that BACP-2 exhibits concentration-dependent developmental toxicity, providing insight into the nanotoxicity of bismuth derivatives, which must be rigorously evaluated with respect to toxicity before their application in nanomedicine.