Bioinspired DNA nanocockleburs for targeted delivery of doxorubicin
Bioinspired DNA nanocockleburs for targeted delivery of doxorubicin
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用于阿霉素靶向递送的仿生 DNA 纳米苍耳
DOI：
10.1016/j.colsurfb.2019.110733
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Yong Jiang
中科院分区：
文献类型：
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作者：
Si Sun;Nihad Cheraga;Han-Ning Jiang;Qian-Ru Xiao;Peng-Cheng Gao;Yang Wang;Ying-Ying Wei;Xiao-Wei Wang;Yong Jiang
各种三维DNA组装体由于其良好的生物相容性和易于制备而被提出作为药物载体。在这项研究中，受苍耳结构的启发，开发了一种新的适配体连接的DNA组装体，用于有效的靶向药物递送。Apt-nanocockleburs通过DNA碱基配对的简单过程制备：应用四条互补的DNA单链（包括一条适体延伸链和三条粘性末端链）彼此配对。nanocockleburs的主体可以装载多柔比星（Dox），而覆盖的适体刺结合到靶MCF-7细胞。自组装的Apt-nanocockleburs比没有适体的DNA nanocockleburs表现出更高的细胞摄取以及对MCF-7细胞的增加的细胞毒性。本研究为制备新型高选择性肿瘤靶向药物载体提供了DNA构建平台。
A variety of three-dimensional DNA assemblies have been proposed as drug carriers owing to their good biocompatibility and easy fabrication. In this study, inspired by the structure of cockleburs, a novel aptamertethered DNA assembly was developed for effective targeted drug delivery. The Apt-nanocockleburs were fabricated via a facile process of DNA base pairing: four complementary DNA single strands, including one aptamerended strand and three sticky-end strands, were applied to pair with each other. The main body of the nanocockleburs can load doxorubicin (Dox) whilst the covered aptamer spines bind to the target MCF-7 cells. The selfassembled Apt-nanocockleburs exhibit higher cell uptake as well as increased cytotoxicity to MCF-7 cells than.DNA nanocockleburs without aptamers. This study provided a DNA constructing platform to produce new drug.carriers with high selectivity for cancer targeted drug delivery.