Polyamine analysis for chemotaxonomy of thermophilic eubacteria:: Polyamine distribution profiles within the orders Aquificales, Thermotogales, Thermodesulfobacteriales, Thermales, Thermoanaerobacteriales, Clostridiales and Bacillales
Polyamine analysis for chemotaxonomy of thermophilic eubacteria:: Polyamine distribution profiles within the orders Aquificales, Thermotogales, Thermodesulfobacteriales, Thermales, Thermoanaerobacteriales, Clostridiales and Bacillales
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DOI：
10.2323/jgam.50.271
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发表时间：
2004-10-01
影响因子：
1.2
通讯作者：
Itoh, T
中科院分区：
文献类型：
--
作者：
Hosoya, R;Hamana, K;Itoh, T
采用高效液相色谱法和气相色谱法对45株嗜热真细菌和8株中温真细菌细胞内多胺的分布进行了研究，探讨了多胺分布的嗜热性和化学分类学意义。亚精胺和一种季铵化的五胺N-4-双（氨丙基）去甲亚精胺是水生目中的嗜热菌属（Thermocrinis）、产氢菌属（Hydrogenophylla）、产氢杆菌属（Hydrogenobaculum）、产液菌属（Aquifex）、Persephonella、产氢硫菌属（Sulfenohydrogenibium）、产氢温菌属（Hydrogenothermus）、浴菌属（Balnearium）和嗜热菌属（Thermovibrio）的主要多胺。属于嗜热芽孢杆菌目的嗜热芽孢杆菌和嗜热脂肪酸菌含有另一种季戊胺，N-4-双（氨丙基）亚精胺。在Thermotogales，Thermotoga含有亚精胺，去甲亚精胺，caldopentamine和homocaldopentamine。后两种线性五胺在Marinitogra和Petrotoga中未发现。在该命令Thermales，Thermus和Marinithermus包含homosperminid，norspermine和线性五胺。Meiothermus缺乏五胺。Vulcanithermus含有线性五胺和六胺，但没有homospermidine。Oceanithermus只含有精胺。在嗜热厌氧细菌目中，在嗜热厌氧单胞菌和嗜热厌氧杆菌中发现了两种季支化五胺。Caldanaerodine含有N-4-双（氨丙基）亚精胺。嗜热厌氧杆菌缺乏五胺。梭菌目的嗜热菌含有N-4-双（氨丙基）亚精胺和胍丁胺。嗜热共生菌属、嗜热空气弧菌属、热臂菌属（梭菌目）、硫杆菌属、脂环酸杆菌属、缺氧杆菌属、脲杆菌属、嗜热菌属（杆菌目）、脱硫肠状菌属、脱硫杆菌属和Pelotomaculum（消化球菌科）普遍含有精胺。芽孢杆菌目的一些嗜热菌添加了直链和支链五胺。
Cellular polyamines of 45 thermophilic and 8 related mesophilic eubacteria were investigated by HPLC and GC analyses for the thermophilic and chemotaxonomic significance of polyamine distribution profiles. Spermidine and a quaternary branched penta-amine, N-4-bis(aminopropyl)norspermidine, were the major polyamine in Thermocrinis, Hydrogenobacter, Hydrogenobaculum, Aquifex, Persephonella, Sulfurihydrogenibium, Hydrogenothermus, Balnearium and Thermovibrio, located in the order Aquificales. Thermodesulfobacterium and Thermodesulfatator belonging to the order Thermodesulfobacteriales contained another quaternary penta-amine, N-4-bis(aminopropyl)spermidine. In the order Thermotogales, Thermotoga contained spermidine, norspermidine, caldopentamine and homocaldopentamine. The latter two linear penta-amines were not found in Marinitogra and Petrotoga. In the order Thermales, Thermus and Marinithermus contained homospermidine, norspermine and the linear penta-amines. Meiothermus lacked penta-amines. Vulcanithermus contained linear penta-amines and hexa-amines but not homospermidine. Oceanithermus contained spermine alone. Within the order Thermoanaerobacteriales, the two quaternary branched penta-amines were found in Thermanaeromonas and Thermoanaerobacter. Caldanaerobacter contained N-4-bis(aminopropyl)spermidine. Thermoanaerobacterium lacked penta-amines. Thermaerobacter of the order Clostridiales contained N-4-bis(aminopropyl)spermidine and agmatine. Thermosyntropha, Thermanaerovibrio, Thermobrachium (the order Clostridiales), Sulfobacillus, Alicyclobacillus, Anoxybacillus, Ureibacillus, Thermicanus (the order Bacillales), Desulfotomaculum, Desulfitobacterium and Pelotomaculum (the family Peptococcaceae) ubiquitously contained spermine. Some thermophiles of Bacillales added linear and branched penta-amines.