Comparison of Nucleotide Sequences of Canker-forming and Non-canker-forming pthA Homologues in Xanthomonas campestris pv. citri
Comparison of Nucleotide Sequences of Canker-forming and Non-canker-forming pthA Homologues in Xanthomonas campestris pv. citri
复制标题
野油菜黄单胞菌中溃疡形成和非溃疡形成 pthA 同源物的核苷酸序列比较。
DOI：
10.3186/jjphytopath.64.462
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
S. Tsuyumu
中科院分区：
文献类型：
--
作者：
H. Kanamori;S. Tsuyumu
野油菜黄单胞菌致病变种（Xanthomonas campestris pv.）与黄单胞菌avrBs3基因家族同源。并测定了它们的核苷酸序列。在这些区域中，从翻译起始点上游247 bp到开放阅读框终止位点下游111 bp的序列是相同的，除了在它们的编码区中间的102 bp的串联重复序列。因此，它们含有相同的亮氨酸拉链，核定位信号和反向重复序列，如De Feyter等人所指出的。其中两个能够使缺失这些avrBs3同源区域之一的非致病性突变体形成溃疡，但剩余的一个甚至在接种后30天都没有。串联重复序列的比较为我们提供了溃疡形成所需的组织结构的更多信息。
Three DNA regions in Xanthomonas campestris pv. citri which were homologous to the avrBs3 gene family commonly found in Xanthomonas spp. were cloned and their nucleotide sequences were determined. The sequences from 247bp upstream of the translational start to 111bp downstream from the termination site of the open reading frames in these regions were identical except in the tandem repeats of 102bp in the middle of their coding regions. Thus, they contain the same leucine zipper, nuclear localizing signals and inverted repeats as noted by De Feyter et al. Two of them enabled a nonpathogenic mutant missing one of these avrBs3 homologous regions to form a canker, but the remaining one did not even 30 days after inoculation. Comparison of the tandem repeats provided us more information on its organization required for canker formation.