Comparison of cell viability and morphology of a human osteoblast-like cell line (SaOS-2) seeded on various bone substitute materials: An in vitro study.
Comparison of cell viability and morphology of a human osteoblast-like cell line (SaOS-2) seeded on various bone substitute materials: An in vitro study.
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DOI：
10.4103/1735-3327.92959
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发表时间：
2012-01
影响因子：
--
通讯作者：
Kharazifar MJ
中科院分区：
文献类型：
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作者：
Ayobian-Markazi N;Fourootan T;Kharazifar MJ
许多研究表明使用不同的骨移植材料有良好的效果。本研究的目的是评估四种不同骨移植材料在体外培养的人成骨样细胞（SaOS-2）的细胞活力和形态方面的生物相容性。研究了Bio-Oss®、Tutodent®、Osteon®和Cerasorb®对人成骨样细胞系的影响，并对各参数进行了评价。将人成骨样细胞植入上述骨替代材料（BSMs）。细胞分化;采用扫描电镜、细胞活力试验和相差显微镜对种子细胞的细胞活力和碱性磷酸酶（ALP）活性进行分析。采用Tamhane事后检验、Kruskal-Wallis检验和Dunn检验。以P<0.05为差异有统计学意义。对照组（SaOS-2细胞在Dulbecco Modified Eagle培养基中孵育，不含任何骨移植材料）的细胞活力最高（P<0.001），其次是Tutodent®、Osteon®、Cerasorb®和Bio-Oss®。Tutodent®与对照组的MTT检测结果无显著差异（P=0.032）。所有骨移植材料的ALP活性均显著高于对照组（P<0.001）。Tutodent组细胞生长最好，Osteon组次之。前者具有较高的纺锤状形貌，与表面有良好的附着力。在Cerasorb®和Bio-Oss®颗粒表面培养的细胞具有更圆的形态。这项体外研究表明，所有测试的bsm都能提供良好的细胞分化，但增殖率较低。
Many studies have shown favorable results following the use of different bone graft materials. The aim of the present study was to evaluate the biocompatibility of four different bone graft materials regarding cell viability and morphology of Human osteoblast-like cells (SaOS-2) in vitro. The effects of Bio-Oss®, Tutodent®, Osteon®, and Cerasorb® were studied on the human osteoblast-like cell line to evaluate various parameters. Human osteoblast-like cells were seeded onto the mentioned bone substitute materials (BSMs). Cell differentiation; cell viability and alkaline phosphatase (ALP) activity of the seeded cells were evaluated by means of scanning electron microscopy, cell viability test and phase contrast microscopy Analysis of variance (ANOVA). Tamhane's post-hoc, Kruskal-Wallis Test, and Dunn's Test were used. The results were considered to be statistically significant at P<0.05. The control group (SaOS-2 cells which were incubated in Dulbecco Modified Eagle Medium without any kind of bone graft materials) had the highest level of cell viability (P<0.001), followed by Tutodent®, Osteon®, Cerasorb®, and Bio-Oss®. There was no significant difference in MTT assay results between Tutodent® and the control group (P=0.032). All tested bone graft materials showed significantly higher ALP activity than the control (P<0.001). The Tutodent® group showed the best cell growth among all experimental groups, followed by the Osteon® group. The former had a higher spindle-like morphology with good attachment to the surface. Cells cultivated on the surfaces of the Cerasorb® and Bio-Oss® granules had more round morphologies. This in vitro study demonstrated that all tested BSMs can provide good cell differentiation but a lower rate of proliferation.