Clinical and microbiological findings in elderly subjects with gingivitis or periodontitis.

Clinical and microbiological findings in elderly subjects with gingivitis or periodontitis.
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患有牙龈炎或牙周炎的老年受试者的临床和微生物学发现。

DOI:
10.1111/j.1600-051x.1998.tb02388.x
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发表时间:
1998
影响因子:
6.7
通讯作者:
Persson,GR
Persson,GR
中科院分区:
医学1区
文献类型:
--
作者:
Schlegel-Bregenzer,B;Persson,RE;Lukehart,S;Braham,P;Oswald,T;Persson,GR

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本研究的目的是通过培养和cDNA探针方法研究20名年龄在62 - 93岁之间的老年受试者的龈上和龈下微生物群落,其中10名仅患有牙龈炎,10名患有牙周炎。牙龈卟啉单胞菌(PG)、中间卟啉单胞菌(PI)、变黑卟啉单胞菌(PN)、A. actinomycetemcomitans(AA)、T.齿垢(TD)和病原体相关的口腔螺旋体(PROS)进行了研究。两组的口腔卫生相似且较差。牙周炎组和牙龈炎组的平均探诊深度分别为6.7mm(S.D. ±1.3)和2.2mm(S.D.±1.5)(F= 17.75,p < 0.001)。平均临床附着水平(CAL)分别为4.3 mm(S.D.±2.0)和1.7 mm(S.D.±0.9)(p< 0.001)。在所有牙周炎患者和8名牙龈炎患者的龈上菌斑样本中发现总活菌计数>1.0× 105,牙周炎组龈上菌斑中发现的黑色色素微生物是对照组的70倍,龈下菌斑中的黑色色素微生物是对照组的185倍(p< 0.01)。10%的牙周炎患者和50%的牙龈炎患者的细菌培养结果显示有P-黑变菌存在(P< 0.03)。在通过Affirm™ DP测试的龈上样本中,BF存在于50%的牙周炎和60%的牙龈炎中,而所有受试者的培养数据均为阴性,PG存在于30%中。牙周炎和50%的牙龈炎受试者中,TD在70%的牙周炎和30%的牙龈炎受试者中。在牙龈菌斑样本中,80%的牙周炎受试者和70%的牙龈炎受试者的厌氧菌>1× 105个。老年牙周炎患者的黑色素微生物总数显著增加(p< 0.001)。通过AffirmTM DP测试的cDNA探针在牙周炎受试者中鉴定出龈下存在BF(80%)、PG(80%)、PI(80%)、AA(0%)、TD(50%),而在牙龈炎受试者中鉴定出BF(70%)、PG(40%)、PI(30%)和TD(20%)。在牙周炎和老年牙龈炎患者中,分别有80%和60%的患者出现PROS。只有PI(r= 0.48,p < 0.01)和TD(r= 0.37,p < 0.01)的量与疾病定义相关。牙周炎组吸烟率明显高于对照组(P< 0.01)。吸烟史可能与牙周炎的致病因素密切相关。
The objective of the present study was to study the supra‐ and subgingival microflora by culture and cDNA probe methods in 20 elderly subjects who were between 62 and 93 years of age, 10 of them had gingivitis only, and 10 had periodontitis.B. forsythus(BF).P. gingivalis (PG), P intermedia (PI), P. nigrescens (PN), A. actinomycetemcomitans (AA), T. denticola (TD), and pathogen‐related oral spirochetes (PROS) were studied. Oral hygiene was similar and poor in both groups. The mean probing depth at sample sites was 6.7 mm (S.D±1.3) in the periodontitis group and 2.2 mm (S.D.±1.5) in the gingivitis group (F=17.75,p< 0.001). Mean clinical attachment levels (CAL) were 4.3 mm (S.D.±2.0) and 1.7 mm (S.D.±0.9) respectively (p< 0.001). Total viable counts >1.0×105in supra‐gingival plaque samples were found in all periodontitis and in eight gingivitis subjects, 70× more black‐pigmented organisms were found in supra‐gingival and 185 times more in sub‐gingival plaque from the periodontitis group (p< 0.01). Culture data showedP nigresecensin 10% periodontitis and 50% gingivitis subjects (p< 0.03). In supra‐gingival samples by the Affirm™ DP test,BFwas present in 50% periodontitis and 60% gingivitis while culture data were negative for all subjects,PGwas found in 30%. periodontitis and 50% gingivitis subjects withTDin 70% periodontitis and in 30% gingivitis subjects. In the ub‐gingival plaque samples 80% periodontitis and 70% gingivitis subjects had >1×105anaerobes. The total count of black‐pigmented organisms was significantly greater in the periodontitis elders (p< 0.001). cDNA probes by the Affirm™ DP test identified subgingival presence ofBF(80%)PG(80%),PI(80%),AA(0%),TD(50%) in periodontitis subjects withBF(70%),PG(40%),PI(30%) andTD(20%) in gingivitis subjects. PROS were found in (80%) samples from periodontitis and in (60%) of gingivitis elderly. Only the quantities ofPI(r=0.48,p< 0.01) andTD(r=0.37,p< 0.01) were associated with the disease definition. The smoking habit in the periodontitis group was significantly higher (p< 0.01). A history of smoking may contribe significantly to periodontitis in the presence of pathogens.
生物行为预防计划的牙周影响。
DOI: --
发表时间: 1998
影响因子: 6.7
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作者:
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DOI: 10.1111/j.1752-7325.1990.tb02131.x
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影响因子: 2.3
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