Apically sorted P2X7 receptors mediate purinergic-induced pore formation preferentially in apical domains of the plasma membrane.
Apically sorted P2X7 receptors mediate purinergic-induced pore formation preferentially in apical domains of the plasma membrane.
复制标题
顶端分选的 P2X7 受体优先在质膜的顶端区域介导嘌呤能诱导的孔形成。
DOI：
10.1080/15257770600890913
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Gorodeski,GI
中科院分区：
文献类型：
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作者：
Li,X;Gorodeski,GI
用P2 X7-受体（P2 X7-R）激动剂BzATP处理附着在滤器上的人宫颈上皮细胞CaSki，诱导了钙的急性瞬时内流，这很可能是P2 X7-R通道激活的结果，随后是较慢的持续钙内流。在溴化乙锭（EB）存在下孵育的培养物中，BzATP诱导缓慢和持续的染料内流，其时间过程与晚期缓慢钙内流相似，表明P2 X7-R孔形成。如果将激动剂添加到管腔溶液中，朝向细胞的顶膜，则BzATP的急性和晚期钙效应更大。BzATP的EB效应最初发生在顶膜，而在基底侧膜的影响是延迟的和较小的。这些结果表明，在极化的上皮细胞在稳态条件下的P2 X7-R位于顶膜，和受体的激活诱导形成的P2 X7-R孔优先在顶膜。
Treatment of human epithelial cervical cells CaSki attached on filters with the P2X7-receptor (P2X7-R) agonist BzATP induced acute transient influx of calcium, most likely the result of P2X7-R channel activation, followed by slower sustained calcium influx. In cultures incubated in the presence of ethidium bromide (EB), BzATP induced slow and sustained influx of the dye with a time-course similar to the late slow calcium influx, suggesting P2X7-R pore formation. The acute and late calcium effects of BzATP were greater if the agonist was added to the luminal solution, facing the apical membrane of the cells. The EB effect of BzATP initially occurred in the apical membrane, while effects in the basolateral membrane were delayed and smaller. These results suggest that in polarized epithelial cells under steady-state conditions the P2X7-R is located in the apical membrane, and activation of the receptor induces formation of P2X7-R pores preferentially in the apical membrane.