Enhancer detection in the ascidian Ciona intestinalis with transposase-expressing lines of Minos
Enhancer detection in the ascidian Ciona intestinalis with transposase-expressing lines of Minos
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DOI：
10.1002/dvdy.21333
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发表时间：
2008-01-01
影响因子：
2.5
通讯作者：
Inaba, Kazuo
中科院分区：
文献类型：
--
作者：
Sasakura, Yasunori;Konno, Aru;Inaba, Kazuo
使用 Tc1/mariner 超家族 Minos 转座子在海鞘中实现了种系转基因。在生殖细胞中表达转座酶的转基因系对于重新动员转座子拷贝非常有用。在本研究中，我们在 Ciona 中创建了 Minos 转座酶表达系。编码鱼精蛋白 (Ci-prm) 的 Ciona 基因在睾丸和精子中表达。使用 Ci-prm 的顺式元件创建在睾丸和精子中表达 Minos 转座酶的转基因系，并用于增强子检测。转座酶系和具有增强子检测载体的转基因系之间的双转基因动物将几个独立的增强子检测事件传递给随后的后代。这项技术使我们能够分离出在受限组织中表达 GFP 的转基因系。该系统为大规模海鞘增强子检测提供了一种简单有效的方法。
Germline transgenesis with a Tc1/mariner superfamily Minos transposon was achieved in the ascidian Ciona intestinalis. Transgenic lines that express transposases in germ cells are very useful for remobilizing transposon copies. In the present study, we created transposase-expressing lines of Minos in Ciona. A Ciona gene encoding protamine (Ci-prm) is expressed in the testes and sperm. Transgenic lines expressing Minos transposase in the testes and sperm were created with a cis-element of Ci-prm, and used for enhancer detection. Double-transgenic animals between transposase lines and a transgenic line with an enhancer detection vector passed on several independent enhancer detection events to subsequent progeny. This technique allowed us to isolate transgenic lines that express GFP in restricted tissues. This system provides an easy and efficient method for large-scale enhancer detection in Ciona intestinalis.