Wax-deficient anther1 is involved in cuticle and wax production in rice anther walls and is required for pollen development
Wax-deficient anther1 is involved in cuticle and wax production in rice anther walls and is required for pollen development
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DOI：
10.1105/tpc.106.042044
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发表时间：
2006-11-01
期刊：
影响因子：
11.6
通讯作者：
An, Gynheung
中科院分区：
文献类型：
--
作者：
Jung, Ki-Hong;Han, Min-Jung;An, Gynheung
在营养叶组织中，包括角质蜡在内的角质层对于防止非气孔失水和病原菌侵染以及适应环境胁迫都是重要的。然而，它们在花药壁中的作用很少被研究。花药壁的最内层(绒毡层)是产生雄配子所必需的。在此，我们报道了水稻蜡质缺陷型花药1(Wda1)基因的T-DNA插入突变体的特征，该突变体在两层中都显示出显著的超长链脂肪酸生物合成缺陷。该基因在花药的表皮细胞中强烈表达。扫描电子显微镜分析表明，花药外层没有表皮层蜡晶，小孢子发育受到严重阻碍，最终由于突变花药中有缺陷的花粉外壁形成而破坏。在wda1突变体中发现的绒毡层的这些生化和发育缺陷比其他雄性不育突变体更早发生，表现为外壁脂类分子的缺陷。我们的发现为蜡质在绒毡层小孢子外壁发育中的作用以及表皮层蜡质在花药展开中的作用提供了新的生化和发育方面的见解。
In vegetative leaf tissues, cuticles including cuticular waxes are important for protection against nonstomatal water loss and pathogen infection as well as for adaptations to environmental stress. However, their roles in the anther wall are rarely studied. The innermost layer of the anther wall (the tapetum) is essential for generating male gametes. Here, we report the characterization of a T-DNA insertional mutant in the Wax-deficient anther1 (Wda1) gene of rice (Oryza sativa), which shows significant defects in the biosynthesis of very-long-chain fatty acids in both layers. This gene is strongly expressed in the epidermal cells of anthers. Scanning electron microscopy analyses showed that epicuticular wax crystals were absent in the outer layer of the anther and that microspore development was severely retarded and finally disrupted as a result of defective pollen exine formation in the mutant anthers. These biochemical and developmental defects in tapetum found in wda1 mutants are earlier events than those in other male-sterile mutants, which showed defects of lipidic molecules in exine. Our findings provide new insights into the biochemical and developmental aspects of the role of waxes in microspore exine development in the tapetum as well as the role of epicuticular waxes in anther expansion.