A coiled-coil protein associates Polycomb Repressive Complex 2 with KNOX/BELL transcription factors to maintain silencing of cell differentiation-promoting genes in the shoot apex
A coiled-coil protein associates Polycomb Repressive Complex 2 with KNOX/BELL transcription factors to maintain silencing of cell differentiation-promoting genes in the shoot apex
复制标题
卷曲螺旋蛋白将 Polycomb Repressive Complex 2 与 KNOX/BELL 转录因子相关联，以维持茎尖细胞分化促进基因的沉默
DOI：
10.1093/plcell/koac133
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Zhou Dao-Xiu
中科院分区：
文献类型：
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作者：
Tan Feng-Quan;Wang Wentao;Li Junjie;Lu Yue;Zhu Bo;Hu Fangfang;Li Qi;Zhao Yu;Zhou Dao-Xiu
多梳阻遏复合物2（PRC 2）介导H3 K27 me 3组蛋白标记的沉积，对动物和植物的发育决策非常重要。在植物茎尖分生组织（SAM）中，三氨基酸环延伸家族（Three Amino Acid Loop Extension family，KNOX/BELL）转录因子是分生组织细胞多能性和分化的关键调控因子。在这里，我们确定了PRC 2相关的卷曲螺旋蛋白（PACP）与KNOX/BELL转录因子在水稻（水稻）茎尖细胞相互作用。PACP的功能缺失突变导致差异基因表达，类似于PRC 2基因敲除植物中观察到的差异基因表达，降低H3 K27 me 3水平，并降低PRC 2核心组分EMF 2b的全基因组结合。PACP的基因组结合显示出与EMF 2b相似的分布模式，具有高PACP和EMF 2b结合信号的基因组区域由高水平的H3 K27 me 3标记。我们表明，PACP是必需的水稻KNOX 1蛋白在SAM靶向细胞分化促进基因的镇压。PACP参与PRC 2向KNOX/BELL转录因子靶向的基因的募集或稳定，以维持H3 K27 me 3和茎尖分裂细胞中的基因阻遏。我们的研究结果提供了深入了解PRC 2介导的H3 K27 me 3的维护和KNOX/BELL蛋白调节SAM发展的机制。
Polycomb repressive complex 2 (PRC2), which mediates the deposition of H3K27me3 histone marks, is important for developmental decisions in animals and plants. In the shoot apical meristem (SAM), Three Amino acid Loop Extension family KNOTTED-LIKE HOMEOBOX /BEL-like (KNOX/BELL) transcription factors are key regulators of meristem cell pluripotency and differentiation. Here, we identified a PRC2-associated coiled-coil protein (PACP) that interacts with KNOX/BELL transcription factors in rice (Oryza sativa) shoot apex cells. A loss-of-function mutation of PACP resulted in differential gene expression similar to that observed in PRC2 gene knockdown plants, reduced H3K27me3 levels, and reduced genome-wide binding of the PRC2 core component EMF2b. The genomic binding of PACP displayed a similar distribution pattern to EMF2b, and genomic regions with high PACP- and EMF2b-binding signals were marked by high levels of H3K27me3. We show that PACP is required for the repression of cell differentiation-promoting genes targeted by a rice KNOX1 protein in the SAM. PACP is involved in the recruitment or stabilization of PRC2 to genes targeted by KNOX/BELL transcription factors to maintain H3K27me3 and gene repression in dividing cells of the shoot apex. Our results provide insight into PRC2-mediated maintenance of H3K27me3 and the mechanism by which KNOX/BELL proteins regulate SAM development.