Rabies virus detection by RT-PCR in decomposed naturally infected brains
Rabies virus detection by RT-PCR in decomposed naturally infected brains
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DOI：
10.1016/s0378-1135(02)00041-x
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发表时间：
2002-06-20
影响因子：
3.3
通讯作者：
Stram, Y
中科院分区：
文献类型：
--
作者：
David, D;Yakobson, B;Stram, Y
以色列温暖的气候和在将身体运往实验室的过程中处理不当，需要一种准确的方法来诊断腐烂组织中的狂犬病。采用逆转录-聚合酶链式反应(RT-PCR)方法，对1998~2000年间采集的10例脑组织标本进行直接荧光抗体试验(FAT)检测，均为阳性。经组织培养狂犬病病毒分离和小鼠接种(MIT)证实，10份腐烂的脑组织中有3份呈阳性，其余7份仅经RT-PCR检测呈阳性。对所有狂犬病病毒N基因的328bp片段进行直接测序和分子分析，证实了它们的地理来源。这些结果证明了RT-PCR在检测自然感染的腐烂脑中狂犬病病毒的重要性，特别是在样品不适合其他实验室检测的情况下。因此，RT-PCR可以提供阳性诊断；然而，当由于技术原因导致腐烂组织的性质而获得阴性结果时，可能会出现假阴性。(C)2002 Elsevier Science B.V.保留所有权利。
The warm climate of Israel and mishandling of the cadavers during transit to the laboratory requires an accurate method for diagnosis of rabies in decomposed tissues. By using the reverse transcriptase polymerase chain reaction (RT-PCR) 10 decomposed brain samples that collected between 1998 and 2000 were diagnosed as negative by direct fluorescent antibody test (FAT), were found positive. Three of the 10 decomposed brains were confirmed as positive by isolation of rabies virus in tissue culture and by mouse inoculation (MIT) while the other seven decomposed samples were found positive only by RT-PCR. Direct sequencing and molecular analysis of a 328 bp fragment of the N gene of all the rabies sequences confirmed their geographical origin. These results demonstrated the importance of the RT-PCR in the detection of rabies virus in decomposed naturally infected brains, especially in cases when the sample is not suitable for other laboratory assays. Thus, the RT-PCR can provide a positive diagnosis; however, when a negative result is obtained due to the nature of the decomposed tissue that can be caused by technical reasons and a false negative might be the case. (C) 2002 Elsevier Science B.V. All rights reserved.