CHARACTERIZATION AND DEVELOPMENTAL EXPRESSION OF BETA-TUBULIN GENES IN DROSOPHILA-MELANOGASTER
CHARACTERIZATION AND DEVELOPMENTAL EXPRESSION OF BETA-TUBULIN GENES IN DROSOPHILA-MELANOGASTER
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DOI：
10.1002/j.1460-2075.1984.tb02170.x
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发表时间：
1984-01-01
期刊：
影响因子：
11.4
通讯作者：
RENKAWITZPOHL, R
中科院分区：
文献类型：
--
作者：
BIALOJAN, S;FALKENBURG, D;RENKAWITZPOHL, R
Genomic clones containing .beta. tubulin sequences were isolated from a .lambda. library of D. melanogaster. In situ hybridization localized 3 genes to 56D and 60B on chromosome 2 as well as to 85D on chromosome 3. The latter was known through genetic analysis to be specifically expressed during spermatogenesis. The genomic clone, pTu85, derived from this region contains one complete .beta. tubulin coding region as well as the 3'' end of an additional so far unidentified .beta. tubulin gene. Genomic Southern hybridizations reveal a total of 5 fragments with .beta. tubulin homology. Clone pTu56 codes for an RNA of 1.8 kb [kilobase] which is expressed in all developmental stages. Clone pTu60 codes for a 2.5-kb RNA expressed during embryogenesis and pupation. In testes RNA a 2.2-kb message homologous to pTu85 was detected.
