Symbiont-Mediated RNA Interference (SMR): Using Symbiotic Bacteria as Vectors for Delivering RNAi to Insects.
Symbiont-Mediated RNA Interference (SMR): Using Symbiotic Bacteria as Vectors for Delivering RNAi to Insects.
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DOI：
10.1007/978-1-0716-1633-8_21
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发表时间：
2022-01-01
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影响因子：
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通讯作者：
Whitten, Miranda M A
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作者：
Dyson, Paul;Figueiredo, Marcela;Whitten, Miranda M A
RNA干扰（RNAi）技术是一种广泛应用于真核生物反向遗传分析的方法。在昆虫中，RNAi也可用于控制昆虫害虫。已经开发了几种方法用于在昆虫中递送干扰RNA，对于不同物种具有不同的结果。在这里，我们描述了如何利用细菌共生体在其昆虫宿主中连续合成干扰双链RNA（dsRNA）。这种称为共生体介导的RNAi（SMR）的方法可以克服与由于唾液或前肠核酸酶的作用而导致的饮食dsRNA的不稳定性相关的问题。由于昆虫不具有可以在其他生物体中扩增和延伸RNAi的RNA依赖性RNA聚合酶活性，SMR还提供了通过其他递送方法将dsRNA单一应用于昆虫所不能提供的长期系统性RNAi的可能性。在这里，我们描述了如何SMR可以应用于全球分布的农业害虫物种，西方花蓟马（Frankliniella oceanentalis）。
RNA interference (RNAi) has emerged as a widely used approach for reverse genetic analysis in eukaryotes. In insects, RNAi also has an application in the control of insect pests. Several methods have been developed for delivery of interfering RNA in insects, with varying outcomes for different species. Here we describe how a bacterial symbiont can be exploited for continuous synthesis of interfering double-stranded RNA (dsRNA) in its insect host. This approach, termed symbiont-mediated RNAi (SMR), can overcome problems associated with instability of dietary dsRNA due to action of salivary or foregut nucleases. As insects do not possess RNA-dependent RNA polymerase activity that can amplify and extend RNAi in other organisms, SMR also offers the possibility of long-term systemic RNAi not afforded by single applications of dsRNA to insects by other delivery methods. Here, we describe how SMR can be applied in a globally distributed agricultural pest species, western flower thrips (Frankliniella occidentalis).