Translation initiation factors and active sites of protein synthesis co-localize at the leading edge of migrating fibroblasts
Translation initiation factors and active sites of protein synthesis co-localize at the leading edge of migrating fibroblasts
复制标题
翻译起始因子和蛋白质合成活性位点共定位于迁移成纤维细胞的前缘
DOI：
10.1042/bj20110435
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发表时间：
2011
影响因子：
4.1
通讯作者：
Willett M
中科院分区：
文献类型：
--
作者：
Willett M
细胞迁移是一个高度受控的基本细胞过程，通常在肿瘤细胞中失控，由细胞的结构动态控制。涉及细胞分离和微阵列图谱的研究先前已经确定了特定于细胞器和建筑隔间的功能不同的mRNA群。然而，翻译机制本身与细胞结构之间的相互作用相对来说还没有被探索。为了帮助理解区域化和定位的蛋白质合成在细胞迁移中的作用，我们使用了扫描共聚焦显微镜、免疫荧光和一种新的核尿肌周期方法来可视化翻译的核糖体。在本研究中，我们发现真核细胞起始因子定位于迁移的MRC5成纤维细胞的前沿，这一过程依赖于TGN(跨高尔基网络)到质膜囊泡的运输。我们发现eIF4E和eIF4GI与高尔基体和膜微域相关，并且这些蛋白中的一部分共定位于迁移细胞前沿的活跃翻译部位。
Cell migration is a highly controlled essential cellular process, often dysregulated in tumour cells, dynamically controlled by the architecture of the cell. Studies involving cellular fractionation and microarray profiling have previously identified functionally distinct mRNA populations specific to cellular organelles and architectural compartments. However, the interaction between the translational machinery itself and cellular structures is relatively unexplored. To help understand the role for the compartmentalization and localized protein synthesis in cell migration, we have used scanning confocal microscopy, immunofluorescence and a novel ribopuromycylation method to visualize translating ribosomes. In the present study we show that eIFs (eukaryotic initiation factors) localize to the leading edge of migrating MRC5 fibroblasts in a process dependent on TGN (trans-Golgi network) to plasma membrane vesicle transport. We show that eIF4E and eIF4GI are associated with the Golgi apparatus and membrane microdomains, and that a proportion of these proteins co-localize to sites of active translation at the leading edge of migrating cells.