Use of quantum dots as mass and fluorescence labels in microarray biosensing
Use of quantum dots as mass and fluorescence labels in microarray biosensing
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DOI：
10.1016/j.talanta.2015.10.012
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发表时间：
2016-01-15
期刊：
影响因子：
6.1
通讯作者：
Chiari, Marcella
中科院分区：
文献类型：
--
作者：
Finetti, Chiara;Plavisch, Lauren;Chiari, Marcella
在这项工作中，我们展示了量子点(QD)质量标记策略在名为干涉反射成像传感器(IRIS)的干涉技术中提高灵敏度的有效性。该生物量检测平台具有绝对质量量化、设备成本低、易于实施等优点。我们讨论了该标记与荧光检测并行使用时的优势。量子点由于其大的可检测质量，在质量标记检测方法中以及在荧光检测中都是一个独特的机会，因为它们不会猝灭地发出荧光。链霉亲和素标记的量子点(SA-Qds)因其大的形状和质量，其655 nm的荧光发射峰，以及对光漂白和猝灭的强烈不敏感性而被研究为这样一种双重作用的探针。特别是，我们探索了它们在微阵列免疫分析中的双重作用，该分析旨在检测针对乳球蛋白的抗体，p-乳球蛋白是一种常见的牛奶过敏原。在饱和条件下，SA-量子点形成了6.2 ng/mm(2)的大的可检测的单分子膜，这一质量信号得到了Platt等人之前的研究的证实。[1]。(C)2015爱思唯尔B.V.保留所有权利。
In this work, we demonstrate the efficacy of a Quantum Dot (QD) mass label strategy to enhance sensitivity in an interferometric technique called interferometric reflectance imaging sensor (IRIS). This biomass detection platform confers the advantage of absolute mass quantification and lower cost, easily implementable equipment. We discuss the advantages of this label when used in parallel with fluorescence detection. QDs represent a unique opportunity to improve sensitivity in both mass-label detection methods due to their large detectable mass, as well as in fluorescence detection, as they fluoresce without quenching. Streptavidin-conjugated QDs (SA-QDs) have been investigated as such a dual-role probe because of their large shape and mass, their 655 nm emission peak for fluorescent detection platforms, and their robust insensitivity to photobleaching and quenching. In particular we explored their dual role in a microarrays immunoassay designed to detect antibodies against p-lactoglobulin, a common milk allergen. The SA-QDs formed a large detectable monolayer of 6.2 ng/mm(2) in the saturation conditions, a mass signal corroborated by previous studies by Platt et al. [1]. (C) 2015 Elsevier B.V. All rights reserved.