Microsatellite loci to recognize species for the cheese starter and contaminating strains associated with cheese manufacturing
Microsatellite loci to recognize species for the cheese starter and contaminating strains associated with cheese manufacturing
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DOI：
10.1016/j.ijfoodmicro.2009.11.014
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发表时间：
2010-02-28
影响因子：
5.4
通讯作者：
Dupont, Joelle
中科院分区：
文献类型：
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作者：
Giraud, Frederic;Giraud, Tatiana;Dupont, Joelle
我们报告了使用富集方案在奶酪真菌 Penicillium comemberti 和 P. roqueforti 中开发了 17 个微卫星标记。使用 23 个 P.cammberti 分离株、26 个 P. roqueforti 分离株以及 A chtysogenum 和 P. nalgiovense 物种各 2 个分离株探索了多态性和交叉扩增。用于生产肉类发酵产品。这些标记似乎对于区分物种很有用，既可以使用它们的扩增大小，也可以使用其侧翼区域的序列。微卫星位点 PC4 特别适合区分与 P.cammberti 密切相关的污染物物种，并阐明该物种与其假定祖先形式 P. commune 的系统发育关系。我们分析了来自不同培养物保藏库、分配给形态种 P 公社的 22 个分离株，其中大多数作为食物破坏者出现，主要来自奶酪环境。它们均未表现出与 P. commune 种的前型分离株相同的序列。相反，它们被分布到另外两个不同的谱系中，对应于旧种 P. fuscoglaucum 和 P. biforme，之前分别与 P. commune 和 P. Cammberti 同义。 P. commune 的前型分离株在所有检查的位点与 P. Camemberti 完全相同。 P. caseifulvum 是一种非产毒物种，被描述为奶酪发酵的新候选者，也表现出与 P. Cammberti 相同的序列。因此，微卫星基因座 PC4 可被视为这些经济上重要物种的条形码的有用候选者。 (C) 2009 Elsevier B.V. 保留所有权利。
We report the development of 17 microsatellite markers in the cheese fungi Penicillium camemberti and P. roqueforti, using an enrichment protocol. Polymorphism and cross-amplification were explored using 23 isolates of P. camemberti, 26 isolates of P. roqueforti and 2 isolates of each of the A chtysogenum and P. nalgiovense species. used to produce meat fermented products. The markers appeared useful for differentiating species, both using their amplification sizes and the sequences of their flanking regions. The microsatellite locus PC4 was particularly suitable for distinguishing contaminant species closely related to P. camemberti and for clarifying the phylogenetic relationship of this species with its supposed ancestral form, P. commune. We analyzed 22 isolates from different culture collections assigned to the morphospecies P commune, most of them occurring as food spoilers, mainly from the cheese environment. None of them exhibited identical sequences with the ex-type isolate of the species P. commune. They were instead distributed into two other distinct lineages, corresponding to the old species P. fuscoglaucum and P. biforme, previously synonymised respectively with P. commune and P. camemberti. The ex-type isolate of P. commune was strictly identical to P. camemberti at all the loci examined. P. caseifulvum, a non toxinogenic species described as a new candidate for cheese fermentation, also exhibited sequences identical to P. camemberti. The microsatellite locus PC4 may therefore be considered as a useful candidate for the barcode of these economically important species. (C) 2009 Elsevier B.V. All rights reserved.