IDENTIFICATION AND SYNTHESIS OF A MAJOR CONSERVED ANTIGENIC EPITOPE OF TRYPANOSOMA-CRUZI
IDENTIFICATION AND SYNTHESIS OF A MAJOR CONSERVED ANTIGENIC EPITOPE OF TRYPANOSOMA-CRUZI
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DOI：
10.1073/pnas.89.4.1239
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发表时间：
1992-02-15
影响因子：
11.1
通讯作者：
REED, SG
中科院分区：
文献类型：
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作者：
BURNS, JM;SHREFFLER, WG;REED, SG
克隆并表达了一个基因序列，该基因编码的免疫显性蛋白具有来自原生动物克氏锥虫（恰加斯病的病原体）的重复表位。鉴定出的10个氨基酸重复序列存在于高分子量锥虫鞭毛虫抗原中，该抗原似乎对锥虫具有特异性且在锥虫中保守。克鲁兹分离物。95%以上的T.包括慢性和急性查加斯病的克氏感染血清含有高水平的抗体，该抗体针对带有重复B细胞表位的15个氨基酸的合成肽。考虑到T. cruzi寄生虫，以及南美锥虫病的广谱临床表现，患者中这种普遍的免疫应答是重要的，并且适用于通过诊断南美锥虫病来控制南美锥虫病。克氏感染
A gene sequence encoding an immunodominant protein with a repetitive epitope from the protozoan Trypanosoma cruzi, the causative agent of Chagas disease, was cloned and expressed. The identified 10-amino acid repeat is present within a high-molecular-weight trypomastigote antigen that appears specific to and conserved among T. cruzi isolates. More importantly, > 95% of T. cruzi infection sera, including both chronic and acute Chagas disease, contained elevated levels of antibody to a 15-amino acid synthetic peptide bearing the repetitive B-cell epitope. Considering the wide diversity of T. cruzi parasites, as well as the broad spectrum of clinical manifestations of Chagas disease, such a prevalent immune response among patients is significant and applicable to the control of Chagas disease through the diagnosis of T. cruzi infection.