The N-glycan profile of mouse myelin, a specialized central nervous system membrane.
The N-glycan profile of mouse myelin, a specialized central nervous system membrane.
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小鼠髓磷脂（一种特殊的中枢神经系统膜）的 N-聚糖谱。
DOI：
10.1111/j.1471-4159.2007.04823.x
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发表时间：
2007
影响因子：
4.7
通讯作者：
Pfeiffer,StevenE
中科院分区：
文献类型：
--
作者：
Ishii,Akihiro;Ikenaka,Kazuhiro;Pfeiffer,StevenE
细胞类型和膜多样性的复杂性阻碍了对细胞膜中糖链丰富补体的理解。为了克服这个问题，我们分析了CNS髓鞘糖蛋白的theN - linked糖链组成，这是少突胶质细胞（OLs）质膜的一种精细结构，导致神经元轴突的多层包裹，促进神经传导，极大地节省了空间和能量。由于通常具有较高的脂蛋白比，髓磷脂可以在蔗糖梯度上很容易地从其他较重的膜中分离出来，并进一步分解成与髓磷脂结构和功能相关的亚域，包括致密髓磷脂和髓磷脂相关的轴胚层膜（Menonet, 2003）。我们使用二维高效液相色谱分析了这些组成n链的糖链，并进行了肼解和吡啶层合。我们的研究结果表明，与全脑匀浆相比，髓磷脂相关的腋膜中n -聚糖的含量高1.3倍，但CM中的n -聚糖含量低0.5倍。M5 [Manα1-3 ((Manα1-3)(Manα1-6) Manα1-6) man β1 - 4glcnac]是脑匀浆、致密髓磷脂和髓磷脂相关轴膜中含量最多的糖链，约占糖链的20%。虽然糖链的类型在馏分中是相似的，但它们的表达水平却有很大的不同。除了高甘露糖型低聚糖外，核心集中的双触角yn‐聚糖具有双分n‐乙酰氨基葡萄糖（GlcNAc）残基，A2G1(3)FB [galβ 1 - 4glcnac β1 - 2man α - 1 - 3(GlcNAcβ1 - 2man α - 6))(GlcNAcβ1 - 4) (GlcNAcβ1 - 2man α - 1 - 3(GlcNAcβ1 - 2man α - 6)GlcNAc], A2G1(6)FB [GlcNAcβ1 - 2man α - 1 - 3(galβ 1 - 4glcnac β1 - 4) (Fucα1-6)GlcNAc]和BA‐1 [man α - 1 - 3(GlcNAcβ1 - 2man α - 6))(GlcNAcβ1 - 4) (GlcNAcβ1 - 4))（GlcNAcβ1 - 4) (GlcNAcβ1 - 4)）和A1(6)G0F [Manα1-3 (GlcNAcβ1 - 2man α1 - 6) Manβ1-4GlcNAcβ1-4 (Fucα1-6) GlcNAc]在致密髓鞘中也有较大比例的存在。我们认为这些差异可能与髓磷脂-腋膜功能有关。
Understanding the rich complement of sugar chains found in cellular membranes is impeded by the complexity of cell types and membrane diversity. To overcome this, we have analyzed theN‐linked sugar chain composition of the glycoproteins of CNS myelin, an elaboration of the plasma membranes of oligodendrocytes (OLs) that result in a multilamellar wrapping of neuronal axons, facilitating nerve conduction with dramatic savings of space and energy. Due to an usually high lipid to protein ratio, myelin can be separated readily from other heavier membranes on sucrose gradients and further fractionated into subdomains related to myelin structure and function, including compact myelin and myelin‐associated axolemmal membrane (Menonet al.2003). We analyzed these fractions forN‐linked sugar chains, using 2D HPLC following hydrazinolysis and pyridylamination. Our results indicate that compared with total brain homogenate, the amount ofN‐glycans is 1.3‐fold higher in the myelin‐associated axolemmal membranes, but it is 0.5‐fold less in CM. M5 [Manα1–3((Manα1–3)(Manα1–6)Manα1–6)Manβ1–4GlcNAcβ1–4GlcNAc] is the most abundant sugar chain in total brain homogenate, compact myelin, and myelin‐associated axolemma, constituting ∼20% of sugar chains. Although the types of sugar chains are similar among the fractions, their expression levels vary significantly. In addition to high mannose type oligosaccharides, the core fucosylated, biantennaryN‐glycans with bisectingN‐acetylglucosamine (GlcNAc) residue, A2G1(3)FB [Galβ1–4GlcNAcβ1–2Manα1–3(GlcNAcβ1–2Manα1–6)(GlcNAcβ1–4)Manβ1–4GlcNAcβ1–4(Fucα1–6)GlcNAc], A2G1(6)FB [GlcNAcβ1–2Manα1–3(Galβ1–4GlcNAcβ1–2Manα1–6)(GlcNAcβ1–4)Manβ1–4GlcNAcβ1–4 (Fucα1–6)GlcNAc] and BA‐1 [Manα1–3(GlcNAcβ1–2Manα1–6)(GlcNAcβ1–4)Manβ1–4GlcNAcβ1–4(Fucα1–6)GlcNAc], and A1(6)G0F [Manα1–3(GlcNAcβ1–2Manα1–6)Manβ1–4GlcNAcβ1–4(Fucα1–6) GlcNAc] are also present in relatively large proportions in compact myelin. We suggest that these differences may be related to myelin‐axolemmal function.