LOCALIZATION OF GLYCINE-CONTAINING NEURONS IN THE MACACA MONKEY RETINA
LOCALIZATION OF GLYCINE-CONTAINING NEURONS IN THE MACACA MONKEY RETINA
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DOI：
10.1002/cne.902730404
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发表时间：
1988-07-22
影响因子：
2.5
通讯作者：
GOEBEL, DJ
中科院分区：
文献类型：
--
作者：
HENDRICKSON, AE;KOONTZ, MA;GOEBEL, DJ
放射自显影后3 H-甘氨酸（Gly）的摄取和免疫细胞化学与Gly特异性抗血清被用来识别猕猴视网膜中含有高水平的这种神经递质的神经元。高亲和力摄取的甘氨酸被证明是钠依赖性的，而释放的内源性和积累的甘氨酸是钙依赖性的。Gly标记的神经元包括40-46%的无长突细胞和近40%的双极细胞。突触标记被认为是整个内丛状层（IPL），但优先分布在内半。S2、S4和S5出现标记斑点带。光和postbedding电子显微镜（EM）免疫细胞化学鉴定不同类型的无长突和双极细胞体和它们的突触末梢。标记最重的Gly+细胞体通常是无长突细胞，具有单个厚的基底树突，延伸深入IPL，在EM水平，电子致密的细胞质和突出的核内折叠。这种细胞类型可以与人视网膜中的Gly 2细胞同源（Marc和Liu：J.Comp.Neurol. 232：241-260，“85）和猫视网膜的AII/Gly 2（Famiglietti和Kolb：Brain Res. 84：293-300，”75; Pourcho和Goebel：J. Comp. Neurol. 233：473-480，'' 85a）. Gly+无长突最常与Gly-无长突以及Gly-和Gly+双极突触。Gly+双极细胞似乎是锥双极细胞，因为它们的标记树突只能追溯到锥蒂。这些标记的树突树的模式表明，弥漫型和侏儒型的双极甘氨酸+。标记突触的EM分布显示Gly+无长突突触遍及IPL，但这些突触仅占无长突群体的11-23%。大多数Gly+双极终末位于内IPL中，其中70%的双极终末被标记。这些研究结果与以前从猫和人类的数据是一致的，表明无长突细胞和双极细胞有助于甘氨酸介导的神经传递在猴子视网膜。
Autoradiography following 3H-glycine (Gly) uptake and immunocytochemistry with a Gly-specific antiserum were used to identify neurons in Macaca monkey retina that contain a high level of this neurotransmitter. High-affinity uptake of Gly was shown to be sodium dependent whereas release of both endogenous and accumulated Gly was calcium dependent. Neurons labeling for Gly included 40-46% of the amacrine cells and nearly 40% of the bipolars. Synaptic labeling was seen throughout the inner plexiform layer (IPL) but with a preferential distribution in the inner half. Bands of labeled puncta occurred in S2, S4, and S5. Both light and postembedding electron microscopic (EM) immunocytochemistry identified different types of amacrine and bipolar cell bodies and their synaptic terminals. The most heavily labeled Gly+ cell bodies typically were amacrine cells having a single, thick, basal dendrite extending deep into the IPL and, at the EM level, electron-dense cytoplasm and prominent nuclear infoldings. This cell type may be homologous with the Gly2 cell in human retina (Marc and Liu: J. Comp. Neurol. 232:241-260, ''85) and the AII/Gly2 of cat retina (Famiglietti and Kolb: Brain Res. 84:293-300, ''75; Pourcho and Goebel: J. Comp. Neurol. 233:473-480, ''85a). Gly+ amacrines synapse most frequently onto Gly- amacrines and both Gly- and Gly+ bipolars. Gly+ bipolar cells appeared to be cone bipolars because their labeled dendrites could be traced only to cone pedicles. The pattern of these labeled dendritic trees indicated that both diffuse and midget types of bipolars were Gly+. The EM distribution of labeled synapses showed Gly+ amacrine synapses throughout the IPL, but these composed only 11-23% of the amacrine population. Most of the Gly+ bipolar terminals were in the inner IPL, where 70% of all bipolar terminals were labeled. These findings are consistent with previous data from cats and humans and suggest that both amacrine and bipolar cells contribute to glycine-mediated neurotransmission in the monkey retina.