Effect of Calcium Chloride Hydrothermal Treatment of Titanium on Protein, Cellular, and Bacterial Adhesion Properties
Effect of Calcium Chloride Hydrothermal Treatment of Titanium on Protein, Cellular, and Bacterial Adhesion Properties
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DOI：
10.3390/jcm9082627
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发表时间：
2020-08-01
影响因子：
3.9
通讯作者：
Koyano, Kiyoshi
中科院分区：
文献类型：
--
作者：
Haraguchi, Takuya;Ayukawa, Yasunori;Koyano, Kiyoshi
牙种植体表面的形貌修饰是材料改进的主要课题之一，然而，粗糙的表面有一些种植体周围炎的风险。据报道，使用氯化钙溶液对钛进行水热处理（HT）可改善骨整合和软组织封闭，而不会改变表面形貌;然而，其机制尚不清楚。因此，我们研究了细胞外基质（ECM）蛋白和HT钛之间的相互作用。此外，我们还阐明了细菌的相互作用。我们采用了两种HT，水HT（DW-HT）和氯化钙溶液HT（Ca-HT）。结果，层粘连蛋白-332和骨桥蛋白在Ca-HT表面的吸附增强。与此相反，白蛋白的吸附，据报道，没有细胞粘附能力，不受Ca-HT。成骨细胞与Ca-HT的粘附也增强。虽然Ca-HT被报道增强上皮细胞附着强度和体内种植体周围上皮结合，但即使在HT后上皮细胞附着的数量也没有增加。Ca-HT对戈登链球菌的粘附无影响。这些结果表明，Ca-HT增强细胞粘附到钛上，而不增加细菌粘附，并且ECM蛋白吸附的改善被认为有助于细胞粘附。
Topographical modification of the dental implant surface is one of the main topics for the improvement of the material, however, the roughened surface has some risks for peri-implantitis. A hydrothermal treatment (HT) of titanium with calcium chloride solution was reported to improve osseointegration and soft tissue sealing without changing the surface topography; however, its mechanism is unclear. We herewith investigated the interaction between extracellular matrix (ECM) protein and HT titanium. Furthermore, we also clarified the bacterial interaction. We employed two kinds of HT, HT with water (DW-HT) and HT with calcium chloride solution (Ca-HT). As a result, the adsorptions of both laminin-332 and osteopontin onto the Ca-HT surface were enhanced. In contrast, the adsorption of albumin, which was reported to have no cell adhesion capacity, was not influenced by Ca-HT. Osteoblast adhesion onto Ca-HT was also enhanced. Although Ca-HT was reported to enhance both epithelial cell attachment strength and in vivo peri-implant epithelial bonding, the number of epithelial cell attachment was not increased even after HT. Ca-HT had no impact in the adhesion ofStreptococcus gordonii. These results suggest that Ca-HT enhances cell adhesion onto titanium without increasing bacterial adhesion, and the improvement of ECM protein adsorption is supposed to contribute to cell adhesion.