VARIABILITY IN THE PRIMARY SITE OF PHOTOSYNTHETIC DAMAGE IN SYMBIODINIUM SP (DINOPHYCEAE) EXPOSED TO THERMAL STRESS
VARIABILITY IN THE PRIMARY SITE OF PHOTOSYNTHETIC DAMAGE IN SYMBIODINIUM SP (DINOPHYCEAE) EXPOSED TO THERMAL STRESS
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DOI：
10.1111/j.1529-8817.2011.01099.x
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发表时间：
2012-02-01
影响因子：
2.9
通讯作者：
Ralph, Peter J.
中科院分区：
文献类型：
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作者：
Buxton, Lucy;Takahashi, Shunichi;Ralph, Peter J.
暴露于高温是已知的，导致光合作用的抑制珊瑚共生体共生藻属。通过使用人工电子受体，甲基紫精，本研究确定了如何减少光合能力发生的结果，抑制铁氧还蛋白的上游和/或下游共生藻属。珊瑚物种和共生体分支之间的异质性被确定在光合作用的温度敏感性的石珊瑚Stylophora pistillata和Pocillopora damicornis的共生体，以及分支A，B，和C的共生藻文化。结果表明，S. pistillata和培养的进化枝C共生藻都表现出相似的模式，因为它们的热抑制的主要位点在32 ℃时发生在铁氧还蛋白的下游。相比之下，在32 ℃时，热抑制的主要位点发生在进化枝A和B中的铁氧还蛋白的上游，而在34 ℃时，所有样品显示组合的上游和下游抑制。虽然C支是两个共同的P damicornis和S。pistillata中的热抑制方式与磷铁矿中的热抑制方式不一致。结果表明，共生藻热损伤的初始部位在珊瑚种和共生体分支之间存在异质性。
Exposure to elevated temperature is known to cause photosynthetic inhibition in the coral symbiont Symbiodinium sp. Through the use of the artificial electron acceptor, methyl viologen, this study identified how reduced photosynthetic capacity occurs as a result of inhibition up- and/or downstream of ferredoxin in Symbiodinium sp. in hospite and in culture. Heterogeneity between coral species and symbiont clades was identified in the thermal sensitivity of photosynthesis in the symbionts of the scleractinian corals Stylophora pistillata and Pocillopora damicornis, as well as among Symbiodinium cultures of clades A, B, and C. The in hospite symbionts of S. pistillata and the cultured clade C Symbiodinium both exhibited similar patterns in that their primary site of thermal inhibition occurred downstream of ferredoxin at 32 degrees C. In contrast, the primary site of thermal inhibition occurred upstream of ferredoxin in clades A and B at 32 degrees C, while at 34 degrees C, all samples showed combined up- and downstream inhibition. Although clade C is common to both P. damicornis and S. pistillata, the manner of thermal inhibition was not consistent when observed in hospite. Results showed that there is heterogeneity in the primal site of thermal damage in Symbiodinium among coral species and symbiont clades.