Electrochemical Biosensors Based on Native DNA and Nanosized Mediator for the Detection of Anthracycline Preparations
Electrochemical Biosensors Based on Native DNA and Nanosized Mediator for the Detection of Anthracycline Preparations
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DOI：
10.1002/elan.201400564
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发表时间：
2015-01-01
期刊：
影响因子：
3
通讯作者：
Budnikov, Herman
中科院分区：
文献类型：
--
作者：
Evtugyn, Gennady;Porfireva, Anna;Budnikov, Herman
研制了一种基于电聚中性红(NR)修饰的玻碳电极(GCE)和聚羧基硫杂环己烷[4]芳烃(共价介体和静电吸附DNA)的新型电化学DNA传感器。阿霉素、柔红霉素和伊达阿比星嵌入DNA后，增加了DNA的电荷转移电阻，减少了电子交换，导致了NR还原的阴极峰的衰减。研制的DNA传感器可以检测0.05 nM阿霉素、0.1 nM阿霉素和0.5 nM伊达比星。该DNA传感器用于药物和人工血浆中阿霉素的检测，回收率为95-100%。
A novel electrochemical DNA-sensor based on glassy carbon electrode (GCE) modified with electropolymerized Neutral red (NR) and polycarboxylated thiacax[4]arene with covalently attached mediator and electrostatically adsorbed DNA was developed for sensitive detection of anthracycline preparations. Intercalation of doxorubicin, daunorubicin and idarubicin into DNA increases the charge transfer resistance and decreases the electron exchange resulted in decay of the cathodic peak of NR reduction. The DNA-sensor developed makes it possible to determine down to 0.05nM doxorubicin, o.1nM doxorubicin and 0.5nM idarubicin. The DNA-sensor was tested on detection of doxorubicin in pharmaceuticals and artificial blood plasma with 95-100% recovery.