Establishment of 3D Intestinal Organoid Cultures from Intestinal Stem Cells
Establishment of 3D Intestinal Organoid Cultures from Intestinal Stem Cells
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DOI：
10.1007/978-1-4939-7021-6_7
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发表时间：
2017-01-01
期刊：
影响因子：
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通讯作者：
Sato, Toshiro
中科院分区：
文献类型：
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作者：
Sugimoto, Shinya;Sato, Toshiro
肠上皮是成年哺乳动物中更新最快的组织，其更新受到肠干细胞的严格控制。使用肠上皮遗传模型的广泛研究揭示了肠干细胞自我更新的机制。利用这些知识，我们开发了一种新颖的 3D 培养系统，使来自小鼠和人体组织的肠道 Lgr5(+) 干细胞能够生长成不断扩大的隐窝绒毛小肠，称为肠上皮类器官。这些类器官通过干细胞的自我更新来维持，并产生肠上皮的所有分化细胞类型。一旦建立，类器官就可以冷冻保存，并在需要时解冻。该培养系统已广泛用于研究干细胞行为和基因功能以及疾病建模。
The intestinal epithelium is the most rapidly renewed tissue in adult mammals, and its renewal is strictly controlled by intestinal stem cells. Extensive studies using genetic models of intestinal epithelium have revealed the mechanisms underlying the self-renewal of intestinal stem cells. Exploiting this knowledge, we developed a novel 3D culture system that enables the outgrowth of intestinal Lgr5(+) stem cells derived from mouse and human tissues into ever-expanding crypt-villus mini-guts, known as intestinal epithelial organoids. These organoids are maintained by the self-renewal of stem cells and give rise to all differentiated cell types of the intestinal epithelium. Once established, organoids can be cryopreserved and thawed when needed. This culture system has been widely used for studying stem cell behavior and gene function and for disease modeling.