WNT signalling molecules act in axis formation in the diploblastic metazoan Hydra
WNT signalling molecules act in axis formation in the diploblastic metazoan Hydra
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DOI：
10.1038/35025063
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发表时间：
2000-09-14
期刊：
影响因子：
64.8
通讯作者：
Holstein, TW
中科院分区：
文献类型：
--
作者：
Hobmayer, B;Rentzsch, F;Holstein, TW
分泌蛋白的Wnt/wingless家族的成员在许多发育组织中的轴形成、器官发生和肿瘤发生期间充当短程诱导物和长程组织者（1）。Wnt信号通路在线虫、昆虫和脊椎动物中是保守的（2）。尽管其发育意义，Wnt信号的进化起源尚不清楚。在这里，我们描述的Wnt信号通路DWnt，Dishevelled，GSK 3，β-连环蛋白和Tcf/左水螅，进化古老的后生动物门刺胞动物的成员的成员的分子特征。Wnt和Tcf在假定的水螅头组织者中表达，即下体的上部。Wnt、β-连环蛋白和Tcf在芽形成和头部再生早期建立头部组织者时转录上调。Wnt和Tcf表达结构域也定义了聚集体中从头模式形成所产生的头部组织者。我们的研究结果表明，Wnt信号可能参与轴水螅的形成，并支持这一想法，它是在早期多细胞动物的轴向分化的进化中心。
Members of the Wnt/wingless family of secreted proteins act as short-range inducers and long-range organizers during axis formation, organogenesis and tumorigenesis in many developing tissues(1). Wnt signalling pathways are conserved in nematodes, insects and vertebrates(2). Despite its developmental significance, the evolutionary origin of Wnt signalling is unclear. Here we describe the molecular characterization of members of the Wnt signalling pathwayDWnt, Dishevelled, GSK3, beta-Catenin and Tcf/Lef-in Hydra, a member of the evolutionarily old metazoan phylum Cnidaria. Wnt and Tcf are expressed in the putative Hydra head organizer, the upper part of the hypostome. Wnt, beta-Catenin and Tcf are transcriptionally upregulated when head organizers are established early in bud formation and head regeneration. Wnt and Tcf expression domains also define head organizers created by de novo pattern formation in aggregates. Our results indicate that Wnt signalling may be involved in axis formation in Hydra and support the idea that it was central in the evolution of axial differentiation in early multicellular animals.