Tenascin immunoreactivity as a prognostic marker in usual interstitial pneumonia
Tenascin immunoreactivity as a prognostic marker in usual interstitial pneumonia
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DOI：
10.1164/ajrccm.154.2.8756830
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发表时间：
1996-08-01
影响因子：
24.7
通讯作者：
Paakko, P
中科院分区：
文献类型：
--
作者：
KaarteenahoWiik, R;Tani, T;Paakko, P
本研究对1981~1995年间51例不同类型的纤维性肺疾病中TN的表达进行了研究。我们的目的是检验普通型间质性肺炎(UIP)肺损伤部位TN积聚是否与预后相关。应用免疫组织化学技术对28例UIP、6例脱屑性间质性肺炎(DIP)、6例结节病、5例外源性过敏性细支气管炎、5例闭塞性细支气管炎机化性肺炎(BOOP)和1例非特异性间质性肺炎患者肺组织中TN的表达进行了研究。除TN免疫组织化学外，还对选定的病例进行免疫电子显微镜和Western blotting研究。对于UIP的预后研究，临床随访信息从患者记录中获得。TN在各型肝纤维化中的表达均升高，尤以UIP为甚。在免疫电子显微镜下，uIP中最显著的标记与胶原纤维和2型肺泡细胞内有关。每个被研究的UIP病例通过Western blotting显示M(R)接近200,000的多肽的反应性。在UIP患者中，TN的表达增加，尤其是在化生的细支气管型上皮下，与生存时间缩短有关。免疫电子显微镜结果支持UIP中TN是由再生的上皮细胞合成的，而不是间质细胞对肺间质炎症的反应。
In this investigation, tenascin (Tn) expression was studied in 51 cases of different types of fibrotic lung disorders originating for years 1981 to 1995. Our aim was to test if accumulation of Tn at the site of lung injury in usual interstitial pneumonia (UIP) could correlate with the prognosis. Lung biopsies taken from 28 patients with UIP, six with desquamative interstitial pneumonia (DIP), six with sarcoidosis, five with extrinsic allergic bronchioloalveolitis, five with bronchiolitis obliterans organizing pneumonia (BOOP), and one with nonspecific interstitial pneumonia were studied for the expression of Tn by using an immunohistochemical technique. In addition to Tn immunohistochemistry, selected cases were also studied by immunoelectron microscopy and Western blotting. For prognostic studies in UIP the clinical follow-up information was obtained from the patient records. The expression of Tn was increased in each type of fibrosis, especially in UIP. In immunoelectron microscopy the most prominent labeling in UIP was found in association with collagen fibers and within the type 2 pneumocytes. Every studied case of UIP showed reactivity for a polypeptide of M(r) approximate to 200,000 by Western blotting. In patients with UIP, increased Tn expression, especially under metaplastic bronchiolar-type epithelium, was associated with a shortened survival time. Immunoelectron microscopic findings support the idea that Tn in UIP is synthesized by the regenerating epithelial rather than interstitial cells in response to pulmonary interstitial inflammation.