Structural segregation of gut microbiota between colorectal cancer patients and healthy volunteers
Structural segregation of gut microbiota between colorectal cancer patients and healthy volunteers
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结直肠癌患者和健康志愿者之间肠道微生物群的结构分离
DOI：
10.1038/ismej.2011.109
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发表时间：
2012-02-01
期刊：
影响因子：
11
通讯作者：
Zhao, Liping
中科院分区：
文献类型：
--
作者：
Wang, Tingting;Cai, Guoxiang;Zhao, Liping
尽管长期以来一直怀疑在人类结直肠癌（CRC）的发展中发挥作用，但CRC患者肠道微生物群的组成尚未得到充分描述。在这项研究中，CRC患者（n = 46）和健康志愿者（n = 56）的粪便细菌多样性通过16 S核糖体RNA基因V3区的454焦磷酸测序进行分析。主成分分析和UniFrac分析均表明两个群体间存在结构分离。通过冗余分析确定了48个操作分类单位（OTU）作为与结构差异显著相关的关键变量。与脆弱拟杆菌密切相关的一个OTU在CRC患者的肠道微生物群中富集，而与普通拟杆菌和单形拟杆菌相关的三个OTU在健康志愿者的肠道微生物群中富集。属于肠球菌属、埃希氏菌属/志贺氏菌属、克雷伯氏菌属、链球菌属和消化链球菌属的总共11种OTU在CRC患者的肠道微生物群中显著更丰富，并且属于罗斯拜瑞氏菌属和毛螺菌科的其他产丁酸盐细菌的5种OTU不太丰富。实时定量PCR通过测量丁酰辅酶A CoA转移酶基因的拷贝数进一步验证了CRC患者肠道微生物群中丁酸盐产生菌的显著减少（Mann-Whitney检验，P
Despite a long-suspected role in the development of human colorectal cancer (CRC), the composition of gut microbiota in CRC patients has not been adequately described. In this study, fecal bacterial diversity in CRC patients (n = 46) and healthy volunteers (n = 56) were profiled by 454 pyrosequencing of the V3 region of the 16S ribosomal RNA gene. Both principal component analysis and UniFrac analysis showed structural segregation between the two populations. Forty-eight operational taxonomic units (OTUs) were identified by redundancy analysis as key variables significantly associated with the structural difference. One OTU closely related to Bacteroides fragilis was enriched in the gut microbiota of CRC patients, whereas three OTUs related to Bacteroides vulgatus and Bacteroides uniformis were enriched in that of healthy volunteers. A total of 11 OTUs belonging to the genera Enterococcus, Escherichia/Shigella, Klebsiella, Streptococcus and Peptostreptococcus were significantly more abundant in the gut microbiota of CRC patients, and 5 OTUs belonging to the genus Roseburia and other butyrate-producing bacteria of the family Lachnospiraceae were less abundant. Real-time quantitative PCR further validated the significant reduction of butyrate-producing bacteria in the gut microbiota of CRC patients by measuring the copy numbers of butyryl-coenzyme A CoA transferase genes (Mann-Whitney test, P