Cotranscriptional Role for Arabidopsis DICER-LIKE 4 in Transcription Termination
Cotranscriptional Role for Arabidopsis DICER-LIKE 4 in Transcription Termination
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DOI：
10.1126/science.1214402
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发表时间：
2012-03-30
期刊：
影响因子：
56.9
通讯作者：
Dean, Caroline
中科院分区：
文献类型：
--
作者：
Liu, Fuquan;Bakht, Saleha;Dean, Caroline
转录终止正在成为基因调控的重要组成部分，对基因组进行分区和最小化转录干扰是必要的。我们已经发现了拟南芥RNA沉默酶DICER-LIKE 4（DCL 4）在内源拟南芥基因FCA转录终止中的作用。DCL 4在3'区直接与FCA染色质结合，并促进典型polyA位点下游结构域中新生转录物的切割。在dcl 4突变体中，所得转录通读触发含有相同3'区的转基因的RNA干扰介导的基因沉默。我们得出结论，DCL 4促进拟南芥FCA基因的转录终止，减少从该位点产生的异常RNA的量。
Transcription termination is emerging as an important component of gene regulation necessary to partition the genome and minimize transcriptional interference. We have discovered a role for the Arabidopsis RNA silencing enzyme DICER-LIKE 4 (DCL4) in transcription termination of an endogenous Arabidopsis gene, FCA. DCL4 directly associates with FCA chromatin in the 3' region and promotes cleavage of the nascent transcript in a domain downstream of the canonical polyA site. In a dcl4 mutant, the resulting transcriptional read-through triggers an RNA interference-mediated gene silencing of a transgene containing the same 3' region. We conclude that DCL4 promotes transcription termination of the Arabidopsis FCA gene, reducing the amount of aberrant RNA produced from the locus.