The final pH of bacteria comprising the predominant flora on sound and carious human root and enamel surfaces
The final pH of bacteria comprising the predominant flora on sound and carious human root and enamel surfaces
复制标题
DOI：
10.1177/00220345960750040201
复制
发表时间：
1996-04-01
影响因子：
7.6
通讯作者：
Kent, R
中科院分区：
文献类型：
--
作者：
vanHoute, J;Lopman, J;Kent, R
在低pH下的产酸似乎是细菌的重要致龋特征。然而，这方面的大多数信息仅涉及少数产酸牙菌斑细菌。因此，糖肉汤中的“最终”pH值被确定为各种口腔细菌。他们的来源是：（1）来自晚期牙根病变（ARL）的龋蚀物质，（2）来自无根龋受试者（SRS）的健全牙根表面的牙菌斑，（3）来自龋齿活跃受试者的“白斑”冠状病变和健全冠状表面的牙菌斑，以及（4）来自无龋受试者的健全冠状表面的牙菌斑。来自组1和组2的菌株（ARL，389株; SRS，358株）先前已鉴定（货车Houte等人，1994），属于优势栽培植物群（PCF）。第3组和第4组的菌株也属于PCF，但未鉴定。所有菌株均被置于4个最终pH类别之一中：< 4.2、4.2 - 4.4、4.4 - 4.6和大于或等于4.6。主要发现：（1）ARL样品中含有大量最终pH < 4.2的菌株（平均百分比为25.7）。它们包括所有的乳酸杆菌和变形链球菌（MS），大多数双歧杆菌菌株和非变形链球菌（非MS），以及约20%的放线菌菌株。相比之下，SRS样品包含的最终pH < 4.2的菌株要少得多（平均百分比为8.4），这些菌株几乎都是非MS的。（2）最终pH < 4.4的微生物分别占ARL和SRS样品的平均百分比为41.5和32.1。(3)来自冠状面的样品中菌株的最终pH分布显示出与第1组和第2组相似的相对于龋齿活性的趋势（第3组与第4组）。我们的研究结果进一步支持了这一概念，即增加的致龋条件与能够在低pH值下产酸的生物体的比例增加有关，并且这种转变涉及MS和乳酸杆菌以外的生物体。
Acidogenesis at low pH appears to be an important bacterial cariogenic trait. However, most information in this regard pertains to only a few of the acidogenic dental plaque bacteria. Therefore, the 'final' pH in sugar broth was determined for a wide variety of oral bacteria. Their source was: (1) carious material from advanced root lesions (ARL), (2) plaque from sound root surfaces of root-caries-free subjects (SRS), (3) plaque from ''white spot'' coronal lesions and sound coronal surfaces of caries-active subjects, and (4) plaque from sound coronal surfaces of caries-free subjects. Strains from groups 1 and 2 (ARL, 389 strains; SRS, 358 strains) were previously identified (van Houte et al., 1994) to the genus/species level and belonged to the predominant cultivable flora (PCF). Strains from groups 3 and 4 also belonged to the PCF but were not identified. All strains were placed in one of 4 final pH categories: < 4.2, 4.2 - 4.4, 4.4 - 4.6, and greater than or equal to 4.6. The main findings were: (1) ARL samples contained many strains with a final pH < 4.2 (mean percentage of 25.7). They included all strains of Lactobacillus and mutans streptococci (MS), most Bifidobacterium strains and non-mutans streptococci (non-MS), and about 20% of the Actinomyces strains. By contrast, SRS samples contained far fewer strains with a final pH < 4.2 (mean percentage of 8.4) which were nearly all non-MS. (2) Organisms with a final pH < 4.4 constituted mean percentages of 41.5 and 32.1 for the ARL and SRS samples, respectively. (3) The final pH distribution of strains in samples from coronal surfaces showed a tendency relative to caries activity (group 3 vs. group 4) similar to that for groups 1 and 2. Our findings further support the concept that increased cariogenic conditions are associated with increased proportions of organisms capable of acidogenesis at a low pH and that this shift involves organisms other than the MS and lactobacilli.