Mapping of UV photoproducts along the human P53 gene.
Mapping of UV photoproducts along the human P53 gene.
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沿着人类 P53 基因绘制紫外光产物图。
DOI：
10.1111/j.1749-6632.1994.tb52840.x
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发表时间：
1994
影响因子：
5.2
通讯作者：
Pfeifer,GP
中科院分区：
文献类型：
--
作者：
Tornaletti,S;Rozek,D;Pfeifer,GP
在哺乳动物细胞DNA序列水平上检测DNA加合物的方法已经发展起来，现在有可能将加合物频率和修复效率与人类癌症相关基因某些核苷酸位置的突变联系起来。在很大比例的人类皮肤癌中发现了p53肿瘤抑制基因的突变。这些突变主要是C到T的转换和CC到TT的双重转换突变，这两种类型的碱基改变是由紫外光诱导的。为了寻找加合物分布与特定p53序列突变之间的可能相关性，我们利用连接介导的聚合酶链式反应(LMPCR)在单碱基分辨率下定位了P53基因沿环丁烷二聚体(CBD)和(6-4)光产物的分布。
Methods to detect DNA adducts at the DNA sequence level in mammalian cells have been developed, and it is now possible to relate adduct frequency and repair efficiency with mutations at certain nucleotide positions in human cancer-relevant genes. Mutations in the p53 tumor suppressor gene have been found in a large proportion of human skin cancers. These mutations are predominantly C to T transitions and CC to TT double transition mutations, two types of base alterations specifically induced by UV light. In order to find possible correlations between adduct distribution and mutations at specific p53 sequences, we have mapped at single-base resolution the distribution of cyclobutane dimers (CBD) and (6-4) photoproducts along the p53 gene in UV-irradiated human skin fibroblasts by ligation-mediated polymerase chain reaction (LMPCR).