Photoelectrochemical imaging of action potentials for single cardiomyocytes through contact force manipulation of organoids
Photoelectrochemical imaging of action potentials for single cardiomyocytes through contact force manipulation of organoids
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DOI：
10.1101/2022.09.05.506608
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发表时间：
2022-09
期刊：
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通讯作者：
Rachel Jacques;Bo Zhou;Emilie Marhuenda;Jon Gorecki;Anirban Das;T. Iskratsch;S. Krause
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文献类型：
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作者：
Rachel Jacques;Bo Zhou;Emilie Marhuenda;Jon Gorecki;Anirban Das;T. Iskratsch;S. Krause
心肌细胞动作电位（AP）的准确监测对于了解疾病传播和新疗法的试验至关重要。膜片钳技术在该领域提供了“黄金标准”测量，但众所周知难以操作，并且仅提供单个细胞的测量。在这里，我们提出了光电化学成像（PEI），结合用于控制心肌细胞类器官和传感器表面之间的接触力的设置，用于以高灵敏度测量AP。该方法通过使用药物进行了验证，结果成功地可视化了AP的预期电生理变化。PEI允许同时监测几个细胞，从而为相邻细胞的电生理相互作用打开了进一步的研究。这种方法将PEI的应用扩展到三维几何形状，并为干细胞研究，药物试验和心脏病建模领域提供了进一步研究AP作用的宝贵工具。
Accurate monitoring of cardiomyocyte action potentials (APs) is essential to understand disease propagation and for trials of novel therapeutics. Patch clamp techniques offer ‘gold standard’ measurements in this field, but are notoriously difficult to operate and only provide measurements of a single cell. Here we propose photoelectrochemical imaging (PEI), in conjunction with a setup for controlling the contact force between the cardiomyocyte organoids and the sensor surface, for measuring APs with high sensitivity. The method was validated through the use of drugs, and the results successfully visualized the expected electrophysiological changes to the APs. PEI allows for several cells to be monitored simultaneously, opening further research to the electrophysiological interactions of adjoining cells. This method expands the applications of PEI to three-dimensional geometries and provides the fields of stem cell research, drug trials and heart disease modelling with an invaluable tool to further investigate the role of APs.