Micropropagation and sea-based nursery growth of selected commercial Kappaphycus species in Penang, Malaysia
Micropropagation and sea-based nursery growth of selected commercial Kappaphycus species in Penang, Malaysia
复制标题
DOI：
10.1007/s10811-019-02003-4
复制
发表时间：
2020-04-01
影响因子：
3.3
通讯作者：
Hurtado, Anicia Q.
中科院分区：
文献类型：
--
作者：
Ali, Majid Khan Majahar;Critchley, Alan T.;Hurtado, Anicia Q.
对海藻日益增长的需求促使海藻产业被指定为马来西亚政府经济转型计划(ETP)国家重点经济区(NKEA)倡议下的第三个入口点项目(EPP)。种植卡拉胶植物已成为一项成功的事业，为一些国家的低收入沿海社区提供了一种有希望的替代生计选择。然而，随着时间的推移，红藻作物的生产力在一些地区下降，原因是从最初被认为具有较高产量潜力但导致品系疲劳或活力丧失的单一、选定的遗传种群获得苗木。为了避免无性系繁殖引起的作物生产力问题，已经开始从Kappassicus的发展和成功的微繁殖中提高种植材料，以支持精选的栽培卡拉胶植物的可持续性。本研究以三种海参(Kappassicus)(K.alvarezii(包括两个菌株--棕色和绿色)、K.maresianus(成熟期)和K.striatus(K.striatus))为材料，研究了在添加陆生植物生长调节剂(PGRs)的情况下，海藻提取液粉(AMPEP K+)作为一种有效的培养基成分为生物刺激剂的最佳使用条件。以L-1AMPEP、K++PGRS 3 mg为最佳组合，将试管苗移栽到海床育苗。盐度和浊度与开阔水域的生长速度呈正相关。使用棕色海藻提取物作为生物刺激剂，并作为成本效益高的培养液的主要成分，对所有四个受试菌株进行微繁殖是非常令人鼓舞的，以至于这种处理方法有可能被推广为经济和商业批量生产新植株(无性幼苗)的通用方案，这是马来西亚海藻养殖业迫切需要的，以充分发挥其潜力。
The increasing demands for seaweeds have promoted the designation of the seaweed industry as the third Entry Point Project (EPP) under the National Key Economic Area (NKEA) initiative of the Economic Transformation Programme (ETP) of the Malaysian government. The farming of carrageenophytes has emerged as a successful enterprise and provides a promising alternative livelihood option for low-income coastal communities in several countries. However, over time, the productivity of the red seaweed crop has declined in some regions due to sourcing of seedlings from single, selected genetic stocks considered to have initially higher yield potential but which resulted in strain fatigue or loss of vigour. To circumvent the crop productivity issues arising from clonal propagation, the raising of planting materials from the development and successful micro-propagation of Kappaphycus has been initiated in order to support the sustainability of selected, farmed carrageenophytes. Three species of Kappaphycus (K. alvarezii (to include 2 strains-brown and green) K. malesianus (aring-aring) and K. striatus) were used in the present study to optimize the use of Ascophyllum Marine Plant Extract Powder (AMPEP K+) which had previously been demonstrated to be effective as a culture medium ingredient, acting as a biostimulant, when applied with the addition of terrestrial plant growth regulators (PGRs). The optimum combination of 3 mg L-1 AMPEP K+ + PGRs was used in out-planting the microplantlets to a sea-based nursery. Salinity and turbidity were found to be positively correlated with growth rates in open water. The use of the brown seaweed-derived extract acting as a biostimulant and as the main ingredient of the cost-effective culture medium for the micro-propagation for all four strains of Kappaphycus tested was highly encouraging, so much so that the treatment has the potential to be promoted as a generic protocol for the economic and commercial mass production of new plantlets (asexual seedlings) which are urgently required for Malaysian seaweed farming to meet its fullest potential.