Anti type I allergic property of Japanese butterbur extract and its mast cell degranulation inhibitory ingredients
Anti type I allergic property of Japanese butterbur extract and its mast cell degranulation inhibitory ingredients
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DOI：
10.1021/jf052994o
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发表时间：
2006-04-19
影响因子：
6.1
通讯作者：
Yoshikawa, M
中科院分区：
文献类型：
--
作者：
Shimoda, H;Tanaka, J;Yoshikawa, M
花粉症是一种影响十分之一日本人口的疾病。在雪松花粉传播的季节，许多患者会出现流鼻涕、抽搐和眼睛发痒等症状。日本毛豆是一种很受欢迎的蔬菜，也是日本为数不多的国产蔬菜之一。以大鼠和RBL-2H3肥大细胞为实验对象，研究了毛冬青地上部分水提物(JBE)的抗I型变态反应作用。在大鼠被动皮肤过敏反应中，单次口服吉贝(1000 mg/kg)可抑制该反应。在IgE致敏的RIBL-2H3细胞中，JIBE(10-100mU/L)可抑制β-己糖苷酶的释放、白三烯C-4/D-4/E-4的合成和肿瘤坏死因子-α的产生。此外，高浓度的JBE(1000 mU g/m L)可抑制组胺(10 MU M)和白三烯D-4(10 NM)所致的豚鼠气管条的收缩。寻找JBE中具有抑制肥大细胞脱颗粒活性的成分是通过抑制FL-己糖苷酶的释放来指导的。分离得到2个马兜铃烷型倍半萜，6个多酚类化合物和2个三萜苷。在这些化合物中，多酚的主要成分呋喃酚酸显示出很强的抑制活性(IC50值=2.1mUg/mL)。因此，在抑制肥大细胞激活和释放的介质引起的直接平滑肌反应的基础上，JBE被发现抑制I型变态反应。
Pollenosis is a disease that affects 1 in 10 of the Japanese population. During the season of cedar pollen dispersal, many patients suffer from symptoms such as sniffling, sternutation, and itching of the eyes. Japanese butterbur is a popular vegetable and is one of the few domestic vegetables in Japan. The anti type I allergic effects of an aqueous ethanol extract from aerial parts of Japanese butterbur (JBE) were evaluated in rats and RBL-2H3 mast cells. In the passive cutaneous anaphylaxis reaction in rats, a single oral treatment of JIBE (1000 mg/kg) was found to suppress the reaction. In IgE-sensitized RIBL-2H3 cells, JIBE (10-100 mu g/mL inhibited P-hexosaminidase release, leukotriene C-4/D-4/E-4 synthesis, and TNF-alpha production. Moreover, a high concentration of JBE (1000 mu g/mL) suppressed smooth muscle constriction induced by histamine (10 mu M) and leukotriene D-4 (10 nM) in a guinea pig trachea strip. The search for components in JBE with an inhibitory activity on mast cell degranulation was guided by inhibition of fl-hexsosaminidase release. Two eremophilane-type sesquiterpenes, six polyphenolic compounds, and two triterpene glycosides were isolated. Of these compounds, fukinolic acid, a principal polyphenol constituent, showed potent inhibitory activity (IC50 value = 2.1 mu g/mL). Consequently, On the basis of its inhibition of mast cell activation and direct smooth muscle reaction induced by released mediators, JBE was found to suppress the type I allergic reaction.