Surveillance of Norovirus Infections in the State of Rio De Janeiro, Brazil 2005-2008
Surveillance of Norovirus Infections in the State of Rio De Janeiro, Brazil 2005-2008
复制标题
DOI：
10.1002/jmv.21831
复制
发表时间：
2010-08-01
影响因子：
12.7
通讯作者：
Miagostovich, M. P.
中科院分区：
文献类型：
--
作者：
Ferreira, M. S. R.;Victoria, M.;Miagostovich, M. P.
在巴西里约热内卢州进行了一项为期四年（2005-2008）的诺如病毒（NoV）监测研究，以证明这些病毒在急性胃肠炎暴发和散发病例中的作用。从胃肠炎患者身上获取了 1,687 份粪便样本； 324 人呈轮状病毒阳性。在其余 1,363 个轮状病毒阴性样本中，本研究对 1,087 个样本进行了 NoV RNA 检测。该研究纳入了市公共卫生中心的267名门诊患者和820名住院患者，其样本是通过公立医院的主动监测获得的。使用 MON 431 434 组简并引物进行 NoV GI 和 GII 检测，通过逆转录 (RT) 和聚合酶链反应 (PCR) 检测粪便样本，NoV 阳性样本为 35.1% (381/1,087)，其中住院患者和门诊患者分别为 30.2% (248/820) 和 49.8% (133/267)。感染 NoV 的儿童粪便中出现粘液、呕吐和发烧的频率明显更高。入院患者中未观察到新冠病毒感染的季节性模式；然而，从流动病例中观察到了两个 NoV 感染高峰，表明在这些时间段内曾发生过疫情。分子特征显示 GII 是最常见的基因组，占所有分析序列的 96.3% (104/108)，GII.4 是最常检测到的基因型 (80.7%)，其次是 GII.6、3、14、7 和 8。还观察到两种 GI 菌株 GI.2 和 GI.3。本研究中描述的暴发和散发病例的数量凸显了在监测实验室中实施新型病毒诊断的必要性。 J. Med。病毒。 82:1442-1448, 2010。(C) 2010 Wiley-Liss, Inc.
A 4-year (2005-2008) norovirus (NoV) surveillance study was conducted in the state of Rio Janeiro, Brazil, to demonstrate the role of these viruses in outbreaks and sporadic cases of acute gastroenteritis. A cohort of 1,687 fecal samples was obtained from patients with gastroenteritis; 324 were rotavirus-positive. Of the remainder 1,363 rotavirus-negative samples, 1,087 samples were tested for NoV RNA in this study. The study enrolled 267 outpatients from Municipal Public Health Centers and 820 inpatients, whose samples were obtained by active surveillance in Public Hospitals. Fecal samples were tested by reverse transcription (RT) followed by polymerase chain reaction (PCR) using the MON 431 434 set of degenerate primers for NoV GI and GII detection, and there were 35.1% (381/1,087) positive samples for NoV, consisting of 30.2% (248/820) and 49.8% (133/267) from inpatient and outpatient, respectively. Children infected by NoV had significantly more frequent mucus in feces, vomiting and fever. No seasonal pattern in NoV infections was observed in patients admitted to hospital; however, two peaks of NoV infections were observed from ambulatory cases, suggesting that there was an occurrence of outbreaks in those time periods. Molecular characterization revealed GII to be the most prevalent genogroup, totaling 96.3% (104/108) of all sequences analyzed, and GII.4 was the genotype detected most frequently (80.7%), followed by GII.6, 3, 14, 7, and 8. Two GI strains, GI.2 and GI.3, were also observed. The number of outbreaks and sporadic cases described in this study highlights the need to implement diagnosis of NoV in surveillance laboratories. J. Med. Virol. 82:1442-1448, 2010. (C) 2010 Wiley-Liss, Inc.