Phylogenic analysis of the genus Leishmania by cytochrome b gene sequencing
Phylogenic analysis of the genus Leishmania by cytochrome b gene sequencing
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DOI：
10.1016/j.exppara.2008.12.013
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发表时间：
2009-04-01
影响因子：
2.1
通讯作者：
Uezato, Hiroshi
中科院分区：
文献类型：
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作者：
Asato, Yutaka;Oshiro, Minoru;Uezato, Hiroshi
在先前的报告（Luyo-Acero等人，2004），我们证明了细胞色素B（Cyt B）基因分析是一种有效的方法，用于分类利什曼原虫属的几个分离株;因此，我们进一步将这种方法应用于其他利什曼原虫物种，以努力提高程序的准确性并构建一个新的遗传树。在这项研究中，共30利什曼原虫和内锥虫WHO参考菌株，临床分离的患者分配到28株（人类和非人类致病物种）和两个物种的内锥虫属进行了分析。如前所述，通过PCR扩增每个样品中的Cyt B基因，然后通过几种引物进行测序。基于计算机软件MEGA v3.1和PAUP* v4.0 Beta获得的结果构建遗传树。目前的遗传树与Lainson和Shaw（1987）提出的传统分类方法几乎相同。但是，它提出了以下建议：（1）排除L。（利什曼原虫）主要来自L。(L)热带复合体;（2）L. tarentolae;（3）蜥蜴属L. (L)hertigi complex和L.（五）equatorensis与内锥虫属（Endotrypanum）相近; (L)enrietti，定义为L（L）mexicana复合体;（5）L（L）turanica和L. (L)阿拉比卡位于远离人类致病性利什曼原虫菌株的区域。因此，Cyt B基因分析适用于分析利什曼原虫属的遗传学，并可用于分离非人类致病性物种与人类致病性物种。(C)2008年爱思唯尔公司All rights reserved.
In a previous report (Luyo-Acero et al., 2004), we demonstrated that cytochrome b (Cyt b) gene analysis is an effective method for classifying several isolates of the genus Leishmania; hence, we have further applied this method to other Leishmania species in an effort to enhance the accuracy of the procedure and to construct a new phylogenic tree. In this study, a total of 30 Leishmania and Endotrypanum WHO reference strains, clinical isolates from our patients assigned to 28 strains (human and non-human pathogenic species) and two species of the genus Endotrypanum were analyzed. The Cyt b gene in each sample was amplified by PCR, and was then sequenced by several primers, as reported previously. The phylogenic tree was constructed based on the results obtained by the computer software MEGA v3.1 and PAUP* v4.0 Beta. The present phylogenic tree was almost identical to the traditional method of classification proposed by Lainson and Shaw (1987). However, it produces the following suggestions: (1) exclusion of L. (Leishmania) major from the L. (L) tropica complex; (2) placement of L. tarentolae in the genus Sauroleishmania; (3) L. (L) hertigi complex and L. (V.) equatorensis close to the genus Endotrypanum; (4) L. (L) enrietti, defined as L (L) mexicana complex, placed in another position; and (5) L (L) turanica and L. (L) arabica are located in an area far from human pathogenic Leishmania strains. Cyt b gene analysis is thus applicable to the analyzing phylogeny of the genus Leishmania and may be useful for separating non-human pathogenic species from human pathogenic species. (C) 2008 Elsevier Inc. All rights reserved.