T-cell dynamics during acute SIV infection
T-cell dynamics during acute SIV infection
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DOI：
10.1097/00002030-200401020-00002
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发表时间：
2004-01-02
期刊：
影响因子：
3.8
通讯作者：
Roederer, M
中科院分区：
文献类型：
--
作者：
Mattapallil, JJ;Letvin, NL;Roederer, M
目的：描述急性SIV感染期间T细胞的动态变化，特别是表型定义的记忆T细胞亚群。设计：T细胞是初始和记忆亚群的异质混合物，通过同时测量CD 4、CD 8、CD 45 RA/RO、CD 11 a、CD 28和CD 27来描述。SIV感染对这些亚群的影响进行测量，以评估在不同的细胞类型的功能变化的影响在pathogenicity.Methods：外周血中获得6只SIV感染的猕猴在多次SIV感染前后，并使用12色流式细胞仪进行分析。结果：急性感染的特点是在B细胞下降引起的初始淋巴细胞减少。在急性期早期，总T细胞计数保持稳定;然而，CD 4细胞计数下降，而CD 8 T细胞增加。CD 4 T细胞的下降是幼稚细胞和记忆细胞下降的结果。CD 4 T细胞的CCR 5+或CD 103+亚群被耗尽，但仅部分解释了CD 4记忆T细胞的下降，这表明急性感染与外周T细胞的快速重新分布有关。初始CD 8细胞计数下降，而记忆CD 8细胞计数增加。的增加正好与血浆病毒血症下降，最初是由CD 27-CD 28-（效应）细胞，随后，主要的表型成为CD 27 + CD 28-，类似于中央记忆cells.Conclusions：一个完整的理解急性SIV或HIV感染期间的T细胞动态需要同时评估的T细胞亚群的广谱。内稳态和相关免疫发病机制的变化不能再简单地通过测量幼稚和记忆T细胞亚群来准确描述。(C)2004年利平科特威廉姆斯威尔金斯。
Objectives: To delineate T-cell dynamics during acute SIV infection, particularly of phenotypically defined memory T cell subsets.Design: T cells are a heterogeneous mix of naive and memory subsets delineated by simultaneously measuring CD4, CD8, CD45RA/RO, CD11a, CD28, and CD27. The effects of SIV infection on these subsets was measured to evaluate the impact of changes in functionally distinct cell types during pathogenesis.Methods: Peripheral blood was obtained from six SIV-infected macaques at multiple times before and after SIV infection and analyzed using 12-color flow cytometry.Results: Acute infection was characterized by an initial lymphopenia caused by a decline in B cells. Total T-cell counts remained steady during the early acute phase; however, CD4 cell counts declined while CD8 T cells increased. The decline in CD4 T cells was a result of a decline in both naive and memory cells. CCR5+ or CD103+ subsets of CD4 T cells were depleted but only partially accounted for the decline of CD4 memory T cells, suggesting that acute infection was associated with a rapid redistribution of T cells from the periphery. Naive CD8 cell counts declined while memory CD8 cell counts increased. The increase coincided with declines in plasma viremia and was made up initially of CD27-CD28- (effector) cells; subsequently, the predominant phenotype became CD27+CD28-, akin to central memory cells.Conclusions: A complete understanding of the T-cell dynamics during acute SIV or HIV infection requires the simultaneous evaluation of a broad spectrum of T-cell subsets. Changes in homeostasis and associated immunopathogenesis can no longer be accurately described simply by measuring naive and memory T-cell subsets. (C) 2004 Lippincott Williams Wilkins.