C-FOS PROTOONCOGENE EXPRESSION IN ASTROCYTES ASSOCIATED WITH DIFFERENTIATION OR PROLIFERATION BUT NOT DEPOLARIZATION
C-FOS PROTOONCOGENE EXPRESSION IN ASTROCYTES ASSOCIATED WITH DIFFERENTIATION OR PROLIFERATION BUT NOT DEPOLARIZATION
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DOI：
10.1016/0169-328x(90)90011-2
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发表时间：
1990-06-01
期刊：
影响因子：
--
通讯作者：
SHARP, FR
中科院分区：
文献类型：
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作者：
HISANAGA, K;SAGAR, SM;SHARP, FR
采用Northern blotting和免疫细胞化学方法检测c-fos原癌基因在培养大鼠新皮层星形胶质细胞中的表达。经表皮生长因子（EGF）、碱性成纤维细胞生长因子（bFGF）、二丁基环AMP （db-cAMP）和磷酸二酯（TPA； 12- o -四癸基磷酸二酯13-乙酸酯）处理后，星形胶质细胞中c-fos mRNA有明显的诱导作用，这些物质可诱导星形胶质细胞增殖或分化。经胎牛血清、EGF、bFGF、db-cAMP和TPA处理后，c-fos蛋白免疫反应性（IR）明显升高。高浓度的钙离子载体A23187对培养的星形胶质细胞具有致死性，也增加了c-fos蛋白- ir。较低浓度的钙离子载体（略微增加Ca2+摄取）、高K+和神经生长因子处理对c-fos表达没有可检测到的影响。这些结果表明，去极化不会诱导星形胶质细胞的c-fos，提示c-fos可能在星形胶质细胞的分化和增殖中发挥作用。
Expression of the c-fos proto-oncogene in rat neocortical astrocytes in culture was examined using Northern blotting and immunocytochemistry. Marked induction of c-fos mRNA in astrocytes was observed after treatment with epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), dibutyryl cyclic AMP (db-cAMP), and phorbol diester (TPA; 12-O-tetra-decanoylphorbol 13-acetate), which are known to induce the proliferation or differentiation of astrocytes. Increase of c-fos protein immunoreactivity (IR) was obtained after treatment with fetal calf serum, EGF, bFGF, db-cAMP and TPA. High concentrations of calcium ionophore A23187, which were lethal to cultured astrocytes, also increased c-fos protein-IR. Treatment with lower concentrations of calcium ionophore (which slightly increase Ca2+ uptake), high K+ and nerve growth factor had no detectable effect on c-fos expression. These results show that depolarization does not induce c-fos in astrocytes and suggest that c-fos may play a role in differentiation and proliferation of astrocytes.