Metabolic engineering of Escherichia coli for 1-butanol production
Metabolic engineering of Escherichia coli for 1-butanol production
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DOI：
10.1016/j.ymben.2007.08.003
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发表时间：
2008-11-01
影响因子：
8.4
通讯作者：
Liao, James C.
中科院分区：
文献类型：
--
作者：
Atsumi, Shota;Cann, Anthony F.;Liao, James C.
与乙醇相比，丁醇作为汽油的替代品具有许多优点，因为它的能量含量更高，疏水性更强。通常，1-丁醇是由梭状芽孢杆菌在混合产品发酵中产生的。为了促进菌株的特异性和生产力的改进，我们在大肠杆菌中设计了一条合成途径，并展示了从这种非本地用户友好的宿主生产1-丁醇。对生产1-丁醇的替代基因和竞争途径缺失进行了评估。结果表明，利用大肠杆菌生产1-丁醇是有希望的。(C)2007 Elsevier Inc.保留所有权利。
Compared to ethanol, butanol offers many advantages as a substitute for gasoline because of higher energy content and higher hydrophobicity. Typically, 1-butanol is produced by Clostridium in a mixed-product fermentation. To facilitate strain improvement for specificity and productivity, we engineered a synthetic pathway in Escherichia coli and demonstrated the production of 1-butanol from this non-native user-friendly host. Alternative genes and competing pathway deletions were evaluated for 1-butanol production. Results show promise for using E. coli for 1-butanol production. (C) 2007 Elsevier Inc. All rights reserved.