Hormonal control of insect epidermal cell commitment in vitro
Hormonal control of insect epidermal cell commitment in vitro
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DOI：
10.1038/259115a0
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发表时间：
1976-01
期刊：
影响因子：
64.8
通讯作者：
L. Riddiford
中科院分区：
文献类型：
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作者：
L. Riddiford
依赖于激素环境，昆虫表皮细胞可以表现出几种分化状态之一，这些分化状态由其分泌的角质层类型来表现。在鳞翅目昆虫中，只要保幼激素（JH）滴度高，表皮就会周期性地分泌幼虫角质层以响应蜕皮激素。当幼虫末期JH升下降时，蜕皮激素引起蛹角质层的分泌。在烟草天蛾，天蛾，蜕皮激素的两个不同的释放是必要的这种变态2，3。第一个触发停止喂养，并导致表皮细胞从一个承诺，使幼虫角质层转换为蛹角质层4。此后，JH不再能阻止蛹分化。第二次蜕皮激素释放开始后2天，并导致蛹角质层的合成。因此，在第一次释放期间，可以研究发生的改变细胞定型的事件，而不受参与该定型表达的那些事件的阻碍。我报告的激素操纵这种细胞的承诺在体外系统。
DEPENDING on the hormonal milieu, the insect epidermal cell can express one of several differentiated states manifest by the type of cuticle it secretes. In the Lepidoptera, as long as the juvenile hormone (JH) titre is high, the epidermis secretes larval cuticle periodically in response to ecdysone. When the JH litre declines at the end of larval life1, ecdysone causes the secretion of pupal cuticle. In the tobacco hornworm,Manduca sexta, two distinct releases of ecdysone are necessary for this metamorphosis2,3. The first triggers the cessation of feeding and causes the epidermal cells to switch from a commitment to make larval cuticle to one for pupal cuticle4. After this time JH can no longer prevent pupal differentiation. The second ecdysone release begins 2 d later and causes pupal cuticle synthesis. Consequently, during the time of the first release it is possible to study the events which occur to alter the cellular commitment unhindered by those events involved in the expression of this commitment. I report the hormonal manipulation of this cellular commitment in anin vitrosystem.