Diffusional spread and confinement of newly exocytosed synaptic vesicle proteins.
Diffusional spread and confinement of newly exocytosed synaptic vesicle proteins.
复制标题
DOI：
10.1038/ncomms9392
复制
发表时间：
2015-09-24
影响因子：
16.6
通讯作者：
Haucke V
中科院分区：
文献类型：
--
作者：
Gimber N;Tadeus G;Maritzen T;Schmoranzer J;Haucke V
神经传递依赖于钙触发的含非肽神经递质的小突触囊泡（SV）与活动区（AZ）的突触前膜的胞吐融合，随后是SV膜的代偿性内吞恢复。在这里，我们研究的扩散命运的新exocytosed SV蛋白在海马神经元的高分辨率延时成像。新的胞吐SV蛋白迅速分散在融合后的第一秒内，直到限制在突触前扣。快速扩散扩散和限制，其次是缓慢的再聚簇SV蛋白在围活性内吞区。限制在突触前扣介导的部分SV蛋白协会与网格蛋白为基础的内吞机制，以限制新exocytosed SV蛋白的扩散蔓延。这些数据表明，扩散，和轴突逃逸的新exocytosed囊泡蛋白，抵消网格蛋白为基础的内吞机制与突触前扩散屏障。 神经传递是由突触囊泡（SV）与活性区附近的质膜融合介导的。在这里，Gimber等人观察到，快速扩散扩散和限制之后，通过SV蛋白与基于网格蛋白的机制的结合，SV蛋白在周活性内吞区缓慢重新聚集。
Neurotransmission relies on the calcium-triggered exocytic fusion of non-peptide neurotransmitter-containing small synaptic vesicles (SVs) with the presynaptic membrane at active zones (AZs) followed by compensatory endocytic retrieval of SV membranes. Here, we study the diffusional fate of newly exocytosed SV proteins in hippocampal neurons by high-resolution time-lapse imaging. Newly exocytosed SV proteins rapidly disperse within the first seconds post fusion until confined within the presynaptic bouton. Rapid diffusional spread and confinement is followed by slow reclustering of SV proteins at the periactive endocytic zone. Confinement within the presynaptic bouton is mediated in part by SV protein association with the clathrin-based endocytic machinery to limit diffusional spread of newly exocytosed SV proteins. These data suggest that diffusion, and axonal escape of newly exocytosed vesicle proteins, are counteracted by the clathrin-based endocytic machinery together with a presynaptic diffusion barrier. Neurotransmission is mediated by synaptic vesicles (SVs) fusion with the plasma membrane near active zones. Here, Gimber et al. observe that rapid diffusional spread and confinement is followed by slow reclustering of SV proteins at the periactive endocytic zone through SV protein association with the clathrin-based machinery.