CHARACTERIZATION OF THE MAJOR REGULATORY ELEMENT UPSTREAM OF THE HUMAN ALPHA-GLOBIN GENE-CLUSTER
CHARACTERIZATION OF THE MAJOR REGULATORY ELEMENT UPSTREAM OF THE HUMAN ALPHA-GLOBIN GENE-CLUSTER
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DOI：
10.1128/mcb.11.9.4679
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发表时间：
1991-09-01
影响因子：
5.3
通讯作者：
HIGGS, DR
中科院分区：
文献类型：
--
作者：
JARMAN, AP;WOOD, WG;HIGGS, DR
人类α -珠蛋白基因复合物的主要正调控活性定位于一个与强红细胞特异性dna酶I超敏位点（HS -40）相关的元件，该位点位于ζ -2-珠蛋白mRNA帽位上游40 kb处。该元素的足迹和凝胶位移分析表明，存在核因子GATA-1的四个结合位点和AP-1一致结合序列对应的两个位点。该区域在结构和特征上类似于-珠蛋白基因座控制区的主要组成部分之一；这与来自表达研究的证据表明HS -40是控制α -珠蛋白基因表达的主要因素。
The major positive regulatory activity of the human alpha-globin gene complex has been localized to an element associated with a strong erythroid-specific DNase I hypersensitive site (HS -40) located 40 kb upstream of the zeta-2-globin mRNA cap site. Footprint and gel shift analyses of the element have demonstrated the presence of four binding sites for the nuclear factor GATA-1 and two sites corresponding to the AP-1 consensus binding sequence. This region resembles one of the major elements of the beta-globin locus control region in its constitution and characteristics; this together with evidence from expression studies suggests that HS -40 is a primary element controlling alpha-globin gene expression.