Inhibitory Effects of Atractylodis Lanceae Rhizoma and Poria on Collagen- or Thromboxane A2-Induced Aggregation in Rabbit Platelets
Inhibitory Effects of Atractylodis Lanceae Rhizoma and Poria on Collagen- or Thromboxane A2-Induced Aggregation in Rabbit Platelets
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DOI：
10.1248/bpb.32.856
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发表时间：
2009-05-01
影响因子：
2
通讯作者：
Nakahata, Norimichi
中科院分区：
文献类型：
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作者：
Nasu, Yuiko;Iwashita, Masaya;Nakahata, Norimichi
神药散 (Jia-Wei-Xiao-Yao-San)、时尺药散 (Dang-Gui-Shao-Yao-San) 和 Toki-shigyaku-ka-goshuyu-shokyo-to (Dang-Gui-Si-Ni-Jia-Wu-Zhu-Yu-Sheng-Jiang-Tang) 是汉方（传统中药）在日本有效用于治疗冷感（Hiesho），但其活性成分及其详细机制尚未阐明。 Hiesho的病因包括末梢血液循环不良和血小板聚集性有助于末梢血液循环；因此，我们利用兔血小板在体外研究了汉方药物对血小板聚集的影响。胶原蛋白和 U46619（一种血栓素 A(2) 受体激动剂）引起兔血小板聚集，体外用 Kami-shoyo-san 和 Toki-shakuyaku-san 预处理血小板可有效抑制这种聚集。然而，Toki-shigyakuka-goshuyu-shokyo-to 并没有显着抑制胶原蛋白或 U46619 诱导的血小板聚集。因此，我们研究了白术和茯苓这两种草药对血小板聚集的影响，这两种草药都包含在神药散和时尺药散中，但不包含在时药药膏中。结果表明，白术抑制胶原诱导的血小板聚集，但不抑制U46619诱导的血小板聚集。茯苓有效抑制U46619诱导的血小板聚集，并部分抑制胶原诱导的血小板聚集。另一方面，苍术和茯苓不抑制肾上腺素/二磷酸腺苷或肾上腺素/血清素诱导的血小板聚集。这些结果表明，两种汉方药物 Kami-shoyo-san 和 Toki-shakuyaku-san 对血小板聚集的抑制可能是改善 Hiesho 的机制之一。此外，白术和茯苓也可能是抑制血小板聚集的草药。
Kami-shoyo-san (Jia-Wei-Xiao-Yao-San), Toki-shakuyaku-san (Dang-Gui-Shao-Yao-San) and Toki-shigyaku-ka-goshuyu-shokyo-to (Dang-Gui-Si-Ni-Jia-Wu-Zhu-Yu-Sheng-Jiang-Tang) are Kampo (traditional Chinese) medicines which are traditionally and effectively used for the treatment of chilly sensation (Hiesho) in Japan, but the active components and their detailed mechanisms have not yet been clarified. Etiologies of Hiesho include poor peripheral blood circulation and platelet aggregability contributes to peripheral blood circulation; therefore, we investigated the effect of Kampo medicines on platelet aggregation using rabbit platelets in vitro. Collagen and U46619, a thromboxane A(2) receptor agonist, caused rabbit platelet aggregation, which was potently inhibited by pretreatment of platelets with Kami-shoyo-san and Toki-shakuyaku-san in vitro. Toki-shigyakuka-goshuyu-shokyo-to, however, did not significantly inhibit collagen- or U46619-induced platelet aggregation. Therefore, we examined the effect on platelet aggregation of two herbal medicines, Atractylodis Lanceae Rhizoma and Poria, both of which are contained in Kami-shoyo-san and Toki-shakuyaku-san but not in Toki-shigyaku-kagoshuyu-shokyo-to. As the results indicate, Atractylodis Lanceae Rhizoma inhibited platelet aggregation induced by collagen but not by U46619. Poria effectively inhibited U46619-induced platelet aggregation and it partially inhibited collagen-induced platelet aggregation. On the other hand, Atractylodis Lanceae Rhizoma and Poria did not inhibit adrenaline/adenosine diphosphate- or adrenaline/serotonin-induced platelet aggregation. These results suggest the possibility that the inhibition of platelet aggregation by two Kampo medicines, Kami-shoyo-san and Toki-shakuyaku-san, is one of the mechanisms underlying the improvement of Hiesho. Furthermore, Atractylodis Lanceae Rhizoma and Poria are possible herbal medicines for the inhibition of platelet aggregation.