Mechanism of inhibition of cytoplasmic streaming by a myosin inhibitor, 2,3-butanedione monoxime
Mechanism of inhibition of cytoplasmic streaming by a myosin inhibitor, 2,3-butanedione monoxime
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DOI：
10.1007/bf01280504
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发表时间：
2000-01-01
期刊：
影响因子：
2.9
通讯作者：
Shimmen, T
中科院分区：
文献类型：
--
作者：
Tominaga, M;Yokota, E;Shimmen, T
在2，3-丁二酮单肟(BDM)对肌球蛋白抑制的基础上，讨论了该蛋白参与多种细胞活动的情况。然而，目前还没有确定BDM是否抑制植物肌球蛋白。本研究分析了BDM对体外分离的植物肌球蛋白的影响。百合(Lilium Long Giflorum)花粉管肌球蛋白与骨骼肌肌动蛋白细丝之间的滑动在60 mM时被抑制25%，表明BDM可作为植物材料的肌球蛋白抑制剂。BDM在30 mM、70 mM短根毛细胞和100 mM长根毛细胞中完全抑制百合花粉管和短根毛细胞的质流。然而。高浓度的BDM诱导百合花粉管和短根毛细胞中肌动蛋白细丝束的解体。此外，BDM处理的短根毛细胞中的皮质微管也碎裂，这可能是BDM的副作用。
On the basis of the inhibition of myosin by 2,3-butanedione monoxime (BDM), the protein's involvement in various cell activities is discussed. However, it has not been established whether BDM inhibits plant myosin. In the present study, the effect of BDM on isolated plant myosin was analyzed in vitro. The sliding between myosin from lily (Lilium longiflorum) pollen tubes and actin filaments from skeletal muscle was inhibited to 25% at a concentration of 60 mM, indicating that BDM can be used as a myosin inhibitor for plant materials. Cytoplasmic streaming was completely inhibited by BDM at 30 mM in lily pollen tubes and at 70 mM in short root hair cells, and at 100 mM in long root hair cells of Hydrocharis dubia. However. BDM at high concentrations induced the disorganization of actin filament bundles in lily pollen tubes and short root hair cells. In addition, cortical microtubules were also fragmented in short root hair cells treated with BDM, suggesting a possible side effect of BDM.