Group 2 innate lymphoid cells can engulf and destroy bacteria
Group 2 innate lymphoid cells can engulf and destroy bacteria
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DOI：
10.1038/s41423-021-00765-x
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发表时间：
2021-09
影响因子：
24.1
通讯作者：
Yiran Yang;Yan Li;Yingjie Xu;Hanxiao Zhang;Yu Diao;Shihao Chen;Ye Cui;C. Corrigan;Wei Wang;Sun Ying
中科院分区：
文献类型：
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作者：
Yiran Yang;Yan Li;Yingjie Xu;Hanxiao Zhang;Yu Diao;Shihao Chen;Ye Cui;C. Corrigan;Wei Wang;Sun Ying
现在已知第2组先天淋巴样细胞（ILC 2）在许多炎症反应中发挥重要作用，涉及Th 2型细胞因子的产生，其有助于寄生虫感染、组织修复和代谢稳态[1]。最近，已经发现ILC 2可以作为抗原呈递细胞，尽管这些细胞的吞噬活性的相应证据目前很少[2，3]。在此，我们通过体外实验研究了ILC 2是否可以吞噬细菌。我们首先从鼻内施用重组小鼠IL-33后的WT小鼠中分离肺ILC 2（定义为谱系-CD 45 + ICOS+ ST 2 + GATA 3+），如前所述[4]（图S1 a，B）。然后，我们使用鼠巨噬细胞系RAW 264。7和人急性T细胞白血病细胞系Jurkat作为阳性和阴性对照，以研究ILC 2的吞噬能力。首先，将不同尺寸（直径50、100 nm和1 μm）的荧光微球加入到RAW 264的培养皿中。7细胞、Jurkat细胞或ILC 2。共聚焦显微镜显示荧光微球位于两种RAW 264的细胞质内。7巨噬细胞和ILC 2（图1a），但不在阴性对照细胞内（图S2 a）。使用共焦断层扫描对这些图像进行三维重建，直观地呈现了上述结果（视频S1和S2）。采用流式细胞术分析来定量由ILC 2和RAW 264进行的各种尺寸的荧光微球的吞噬量。7个细胞。几乎所有的ILC 2都可以吞噬50 nm的荧光微球，尽管效率低于
Group 2 innate lymphoid cells (ILC2s) are now known to play prominent roles in many inflammatory responses involving the production of Th2-type cytokines, which contribute to parasitic infections, tissue repair, and metabolic homeostasis [1]. More recently, it has emerged that ILC2s may act as antigen-presenting cells, although corresponding evidence of phagocytic activity of these cells is currently scarce [2, 3]. Herein, we investigated whether ILC2s can phagocytose bacteria, through in vitro experiments.We first isolated pulmonary ILC2s (defined as lineage− CD45+ ICOS+ ST2+ GATA3+) from WT mice following intranasal application of recombinant mouse IL-33 as previously described [4](Fig. S1a, b). Then, we used the murine macrophage cell line RAW264. 7 and human acute T-cell leukemia cell line Jurkat as the positive and negative controls to investigate the phagocytic ability of ILC2s. Initially, fluorescent microspheres of different sizes (50, 100nm, and 1 μm diameter) were added to culture dishes of RAW264. 7 cells, Jurkat cells or ILC2s. Confocal microscopy revealed that the fluorescent microspheres were located within the cytoplasm of both RAW264. 7 macrophages and ILC2s (Fig. 1 a) but not within negative control cells (Fig. S2a). Three-dimensional reconstruction of these images using confocal tomography intuitively presented the above results (Videos S1 and S2). Flow cytometric analysis was employed to quantify the amount of phagocytosis of the various sizes of fluorescent microspheres performed by the ILC2s and RAW264. 7 cells. Almost all ILC2s could phagocytose 50nm fluorescent microspheres, although less efficiently than