Blockade of the interleukin-2 receptor by anti-Tac antibody inhibits the generation of antigen-nonspecific suppressor T cells in vitro.
Blockade of the interleukin-2 receptor by anti-Tac antibody inhibits the generation of antigen-nonspecific suppressor T cells in vitro.
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抗 Tac 抗体阻断白细胞介素 2 受体可抑制体外抗原非特异性抑制性 T 细胞的产生。
DOI：
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发表时间：
1988
影响因子：
11.1
通讯作者：
Thomas A. Waldmann
中科院分区：
文献类型：
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作者：
Tsutomu Oh;C. Goldman;J. Misiti;Thomas A. Waldmann
用抗Tac单抗阻断IL-2与高亲和力IL-2受体55 kDa多肽的结合，研究IL-2在抑制性T细胞活化中的作用。将抗Tac加入到抗原非特异性抑制系统中，在该系统中，ConA诱导的抑制T细胞在预培养期间产生，并在含有商陆有丝分裂原和外周血单核细胞的第二指示培养中评估它们对免疫球蛋白产生的影响。预培养期间加入抗Tac可抑制ConA诱导的抑制性T细胞生成。经短期(2天)预培养激活成为抑制效应的细胞主要是Tac阳性、T8(CD8)阳性的表型。TAC阳性、T8阴性的T细胞也可能参与了抑制活性。我们的研究表明，抗Tac通过对人的高亲和力IL-2受体产生功能性阻断，抑制抗原非特异性抑制T细胞的产生。
The role of interleukin 2 (IL-2) in the activation of suppressor T cells was investigated by using the monoclonal antibody anti-Tac, which blocks the binding of IL-2 to the 55-kDa peptide of the high-affinity IL-2 receptor. Anti-Tac was added to an antigen-nonspecific suppressor system in which Con A-induced suppressor T cells were generated during a preculture period, and their effects on immunoglobulin production were assessed in second, indicator cultures containing pokeweed mitogen and peripheral blood mononuclear cells. Anti-Tac added during the preculture period inhibited Con A-induced suppressor T-cell generation. Cells activated by a short (2-day) preculture period to become effectors of suppression were primarily of the Tac-positive, T8 (CD8)-positive phenotype. Tac-positive, T8-negative T cells might also contribute to the suppressor activity. Our studies indicate that anti-Tac, by producing a functional blockade of human high-affinity IL-2 receptors, inhibits the generation of antigen-nonspecific suppressor T cells.