The essential YycFG two-component system controls cell wall metabolism in Bacillus subtilis
The essential YycFG two-component system controls cell wall metabolism in Bacillus subtilis
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DOI：
10.1111/j.1365-2958.2007.05782.x
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发表时间：
2007-07-01
影响因子：
3.6
通讯作者：
Devine, Kevin M.
中科院分区：
文献类型：
--
作者：
Bisicchia, Paola;Noone, David;Devine, Kevin M.
细菌对主要环境和营养条件的适应通常由双组分信号转导系统（TCS）介导。枯草芽孢杆菌YycFG TCS引起了特别的关注，因为它是生存力所必需的，并且其调节子的定义很差。在这里，我们表明YycFG是细胞壁代谢的调节剂。我们已经确定了YycFG调节子的五个新成员：YycF激活yvcE，lytE和ydiM的表达，并抑制yoeB和yjeA的表达。YvcE（CwIO）和LytE编码分别参与肽聚糖合成和周转的内肽酶型自溶素。我们表明，一个yvcE lytE双突变株是不可行的，细胞缺乏LytE和耗尽YvcE表现出缺陷，在侧细胞壁合成和细胞伸长。YjeA编码肽聚糖脱乙酰酶，其修饰肽聚糖，从而改变其对溶菌酶消化的敏感性，并且YdjM也被预测在细胞壁代谢中具有作用。遗传分析表明，YycFG的本质是多基因的性质，是一个破坏细胞壁代谢的表现所造成的异常表达的YycFG调节子基因的数量。
Adaptation of bacteria to the prevailing environmental and nutritional conditions is often mediated by two-component signal transduction systems (TCS). The Bacillus subtilis YycFG TCS has attracted special attention as it is essential for viability and its regulon is poorly defined. Here we show that YycFG is a regulator of cell wall metabolism. We have identified five new members of the YycFG regulon: YycF activates expression of yvcE, lytE and ydiM and represses expression of yoeB and yjeA. YvcE(CwIO) and LytE encode endopeptidase-type autolysins that participate in peptidoglycan synthesis and turnover respectively. We show that a yvcE lytE double mutant strain is not viable and that cells lacking LytE and depleted for YvcE exhibit defects in lateral cell wall synthesis and cell elongation. YjeA encodes a peptidoglycan deacetylase that modifies peptidoglycan thereby altering its susceptibility to lysozyme digestion and YdjM is also predicted to have a role in cell wall metabolism. A genetic analysis shows that YycFG essentiality is polygenic in nature, being a manifestation of disrupted cell wall metabolism caused by aberrant expression of a number of YycFG regulon genes.