Recombinant inbred lines derived from cultivars of pea for understanding the genetic basis of variation in breeders' traits
Recombinant inbred lines derived from cultivars of pea for understanding the genetic basis of variation in breeders' traits
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DOI：
10.1017/s1479262118000345
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发表时间：
2018-10-01
影响因子：
1.1
通讯作者：
Domoney, Claire
中科院分区：
文献类型：
--
作者：
Moreau, Carol;Knox, Maggie;Domoney, Claire
为了了解豌豆育种家感兴趣的性状的遗传基础，根据表型差异和遗传差异，选择豌豆品种Brutus、Enigma和Kahuna，构建重组自交系群体。三个组合中的每一个都进行了正反交，每个组合中有200多个F-2种子前进到F-13。F-7种子被用来产生F-8-F(11)群体，这些群体在随机田间试验中在三个地块中种植，并用于收集多个生长季节的表型数据，包括种子重量和产量性状。从F-6世代开始构建遗传图谱，以支持质量和数量性状的分析，并导致确定了与种子重量决定有关的四个主要遗传座位和至少一个负责产量变异的主要基因座位。三个种子重量基因座，其中至少有一个以前没有描述过，与骨髓脂肪种子表型相关。对于一些已识别的基因座，候选基因已经被识别出来。F-13单种子后代系可作为豆科和豆类作物群体的种质资源。
In order to gain an understanding of the genetic basis of traits of interest to breeders, the pea varieties Brutus, Enigma and Kahuna were selected, based on measures of their phenotypic and genotypic differences, for the construction of recombinant inbred populations. Reciprocal crosses were carried out for each of the three pairs, and over 200 F-2 seeds from each cross advanced to F-13. Bulked F-7 seeds were used to generate F-8-F(11 )bulks, which were grown in triplicated plots within randomized field trials and used to collect phenotypic data, including seed weight and yield traits, over a number of growing seasons. Genetic maps were constructed from the F-6 generation to support the analysis of qualitative and quantitative traits and have led to the identification of four major genetic loci involved in seed weight determination and at least one major locus responsible for variation in yield. Three of the seed weight loci, at least one of which has not been described previously, were associated with the marrowfat seed phenotype. For some of the loci identified, candidate genes have been identified. The F-13 single seed descent lines are available as a germplasm resource for the legume and pulse crop communities.