Enhancing the production of astaxanthin by mixotrophic cultivation of Haematococcus pluvialis in open raceway ponds
Enhancing the production of astaxanthin by mixotrophic cultivation of Haematococcus pluvialis in open raceway ponds
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DOI：
10.1007/s10499-019-00483-2
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发表时间：
2019-12
影响因子：
2.9
通讯作者：
Xiaobin Wen;Zhongjie Wang;Yi Ding;Yahong Geng;Yeguang Li
中科院分区：
文献类型：
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作者：
Xiaobin Wen;Zhongjie Wang;Yi Ding;Yahong Geng;Yeguang Li
混合营养培养红球藻可有效提高虾青素产量。然而，由于细菌污染，该技术在开放式生物反应器中的应用受到限制。本研究在室外跑道池塘中成功地进行了红球藻的混合营养养殖。红球藻细胞首先在光照下生长，然后在去除培养液中的硝酸盐后再添加醋酸盐/冰醋酸。在这种条件下，红球藻细胞生长在细胞内的氮池中，而细菌的繁殖受到限制。虾青素的平均产量达到140mgm−2day−1，约为单纯光营养培养的1.2倍。本研究探索了一条提高虾青素产量的潜在途径，其结果对红球藻产业的发展具有一定的指导意义。
Mixotrophic cultivation ofHaematococcusis efficient to increase astaxanthin productivity. However, application of this technique in open bioreactors is restricted because of bacterial contamination. In this study, mixotrophic cultivation ofHaematococcuswas carried out successfully in an outdoor raceway pond. TheHaematococcuscells grew on light first, and then acetate/acetic acid was supplemented when nitrate was depleted from the culture media. Under such conditions,Haematococcuscells grew on intracellular nitrogen pool, while bacterial reproduction was limited. The average astaxanthin productivity reached 140 mg m−2day−1, which is about 1.2 times that of the simple phototrophic cultivation. This study explores a potential way to enhance astaxanthin productivity, and the results are helpful to the development of theHaematococcusindustries.